Changes in intracellular metabolite pools, and acetate formation in Escherichia coli are associated with a cell-density-dependent metabolic switch
Changes in intracellular metabolite pools, and acetate formation in Escherichia coli are associated with a cell-density-dependent metabolic switch
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DOI：
10.1023/a:1014586205347
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发表时间：
2002-03-01
影响因子：
2.7
通讯作者：
Häggström, L
中科院分区：
文献类型：
--
作者：
Han, L;Enfors, SO;Häggström, L
氨基酸代谢中的细胞密度依赖性代谢开关发生在E。大肠杆菌W3110分批培养物（Han L，Doverskog M，Enfors S-O，Haggstrom L，2002，J. Biotechnol. 92：237-249）。大多数糖酵解和柠檬酸循环代谢物的浓度降低2 - 3倍，并且转换后乙酰辅酶A浓度增加，表明中枢代谢也受到影响。在整个培养过程中，比乙酸盐生产速率降低，除了在转换点暂时增加。细胞内乙酸盐浓度在培养期间保持相对恒定。
A cell density-dependent metabolic switch in amino acid metabolism occurs in E. coli W3110 batch cultures at 1.15 g dry wt l(-1) (Han L, Doverskog M, Enfors S-O, Haggstrom L, 2002, J. Biotechnol. 92: 237-249). A two- to three-fold decrease of the concentration of most glycolytic and citric acid cycle metabolites, and an increase in acetyl-CoA concentration after the switch, indicates that the central metabolism also is affected. The specific acetate production rate decreases throughout the culture, except for a temporary increase at the switch point. The intracellular acetate concentration remains relatively constant during the culture.