Nanoreactors by Programmed Enzyme Encapsulation Inside the Capsid of the Bacteriophage P22
Nanoreactors by Programmed Enzyme Encapsulation Inside the Capsid of the Bacteriophage P22
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DOI：
10.1021/nn300545z
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发表时间：
2012-06-01
期刊：
影响因子：
17.1
通讯作者：
Douglas, Trevor
中科院分区：
文献类型：
--
作者：
Patterson, Dustin P.;Prevelige, Peter E.;Douglas, Trevor
源自噬菌体 P22 的病毒样颗粒 (VLP) 提供了一个独特的平台，可通过将酶封装到其内部来构建催化功能纳米材料。封装已被设计为基因编程，允许“一锅”合成和掺入所需的酶。 P22 与其他 VLP 系统的独特之处在于能够调节 VLP 结构的整体体积和孔隙率，从而控制底物与封装酶的接触。本研究表明，乙醇脱氢酶 D 的掺入是迄今为止所描述的任何 VLP 中活性酶内部负载最高的。此外，我们发现，与“游离”酶相比，将 AdhD 封装在 P22 内不仅会影响其动力学参数，而且 P22 向不同形态状态的转变会改变 VLP 的内部体积，也会导致封装酶的整体活性发生变化。这里报告的发现清楚地表明，P22 具有通过设计、利用高度进化的酶的力量进行化学转化来创建纳米反应器的合成方法的潜力。
The virus like particle (VLP) derived from bacteriophage P22 presents a unique platform for constructing catalytically functional nanomaterials by encapsulation of enzymes into its interior. Encapsulation has been engineered to be genetically programmed allowing "one pot" synthesis and incorporation of desired enzymes. The unique characteristic that separates P22 from other VLP systems is the ability to modulate the overall volume and porosity of the VLP structure, thus controlling substrate access to the encapsulated enzyme. The present study demonstrates incorporation of an enzyme, alcohol dehydrogenase D, with the highest internal loading for an active enzyme by any VLP described thus far. In addition, we show that not only does encapsulating AdhD inside P22 affect its kinetic parameters in comparison with the "free" enzyme, but transformation of P22 to different morphological states, which changes the internal volume of the VLP, yields changes in the overall activity of the encapsulated enzyme as well. The findings reported here clearly illustrate that P22 holds potential for synthetic approaches to create nanoreactors, by design, using the power of highly evolved enzymes for chemical transformations.