CORTICAL PROJECTION PATTERNS OF THE MEDIAL SEPTUM-DIAGONAL BAND COMPLEX
CORTICAL PROJECTION PATTERNS OF THE MEDIAL SEPTUM-DIAGONAL BAND COMPLEX
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DOI：
10.1002/cne.902930109
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发表时间：
1990-03-01
影响因子：
2.5
通讯作者：
TRABER, J
中科院分区：
文献类型：
--
作者：
GAYKEMA, RPA;LUITEN, PGM;TRABER, J
通过顺行运输菜豆白细胞凝集素 (PHA-L)，对内侧隔膜-对角带 (MS/DB) 复合体的皮质投影进行了详细分析。将示踪剂通过离子电渗疗法注射到内侧隔膜（MS）和 Broca 对角线带的垂直和水平肢（VDB 和 HDB）的细胞群中，并对轴突内运输的 PHA-L 切片进行免疫组织化学处理。标记的传出信号显示出皮质地幔区域分布的显着差异，这取决于 MS/DB 复合体中注射部位的位置，揭示了 MS/DB 皮质投影的地形组织。简而言之，HDB 的侧面和中间部分，也称为大细胞视前区，主要投射到嗅觉核和外侧内嗅皮层。 HDB 的内侧部分和相邻的 VDB 尾部（有角）部分的特征是向内侧中脑边缘、枕叶和外侧内嗅皮质、嗅球以及皮下和海马区域的背侧广泛、丰富的投射。 VDB 的吻内侧部分和 MS 的投射主要针对整个海马和海马后区域，其次是内侧中脑边缘皮层。此外，MS 投射受到清晰的中外侧地形排列的影响，使得外侧 MS 主要投射到下皮复合体和海马的腹侧/颞侧以及内嗅皮质的内侧部分，而 MS 中更多位于内侧的细胞支配海马和下皮层的更多间隔/背侧部分以及内嗅皮层的更多外侧部分 皮质。已观察到 PHA-L 填充的轴突通过许多途径，即穹窿伞系统、胼胝体上纹、嗅脚和外侧梨状途径（后两者主要通过 HDB 和尾部 VDB）。一般来说，标记的投影分布在所有皮质层中，尽管在几个目标区域中存在清晰的层压图案。丰富的分枝纤维富含“经过的纽扣”和末端纽扣。前额叶、扣带皮层和枕叶皮质中标记的基底前脑传出神经的分布和形态与胆碱乙酰转移酶的免疫组织化学演示所揭示的胆碱能神经支配的分布和形态非常相似。相反，嗅觉、海马、皮下和内嗅区域的标记投影显示出异质形态。在这里，只有薄的静脉曲张投影的分布类似于胆碱能纤维的分布。
A detailed analysis of the cortical projections of the medial septum-diagonal band (MS/DB) complex was carried out by means of anterograde transport of Phaseolus vulgaris leucoagglutinin (PHA-L). The tracer was injected iontophoretically into cells groups of the medial septum (MS) and the vertical and horizontal limbs of the diagonal band of Broca (VDB and HDB), and sections were processed immunohistochemically for the intra-axonally transported PHA-L. The labeled efferents showed remarkable differences in regional distribution in the cortical mantle dependent on the position of the injection site in the MS/DB complex, revealing a topographic organization of the MS/DB-cortical projection. In brief, the lateral and intermediate aspects of the HDB, also referred to as the magnocellular preoptic area, predominantly project to the olfactory nuclei and the lateral entorhinal cortex. The medial part of the HDB and adjacent caudal (angular) part of VDB are characterized by widespread, abundant projections to medial mesolimbic, occipital, and lateral entorhinal cortices, olfactory bulb, and dorsal aspects of the subicular and hippocampal areas. Projections from the rostromedial part of the VDB and from the MS are preponderantly aimed at the entire hippocampal and retrohippocampal regions and to a lesser degree at the medial mesolimbic cortex. Furthermore, the MS projections are subject to a clear mediolateral topographic arrangement, such that the lateral MS predominantly projects to the ventral/temporal aspects of the subicular complex and hippocampus and to the medial portion of the entorhinal cortex, whereas more medially located cells in the MS innervate more septal/dorsal parts of the hippocampal and subicular areas and more lateral parts of the entorhinal cortex. PHA-L filled axons have been observed to course through a number of pathways, i.e., the fimbria-fornix system, supracallosal stria, olfactory peduncle, and lateral piriform route (the latter two mainly by the HDB and caudal VDB). Generally, labeled projections were distributed throughout all cortical layers, although clear patterns of lamination were present in several target areas. The richly branching fibers were abundantly provided with both "boutons en passant" and terminal boutons. Both distribution and morphology of the labeled basal forebrain efferents in the prefrontal, cingulate, and occipital cortices closely resemble the distribution and morphology of the cholinergic innervation as revealed by immunohistochemical demonstration of choline acetyltransferase. In contrast, the labeled projections to the olfactory, hippocampal, subicular, and entorhinal areas showed a heterogeneous morphology. Here, the distribution of only the thin varicose projections resembled the distribution of cholinergic fibers.