Retrovirus-like end processing of the tobacco Tnt1 retrotransposon linear intermediates of replication
Retrovirus-like end processing of the tobacco Tnt1 retrotransposon linear intermediates of replication
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DOI：
10.1128/jvi.71.5.4005-4015.1997
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发表时间：
1997-05-01
影响因子：
5.4
通讯作者：
Lucas, H
中科院分区：
文献类型：
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作者：
Feuerbach, F;Drouaud, J;Lucas, H
烟草反转录转座子Tnt1可以通过RNA中间体在异源宿主拟南芥中转座。我们在这里报告的识别和表征的染色体外线性和环状DNA形式的Tnt1在这个异源宿主。我们的研究结果表明，Tnt1线性中间体具有两个额外的碱基对在每一端相比，Tnt1的整合形式。在整合到宿主基因组中之前，去除这些线性中间体3'末端的两个末端核苷酸，如酵母Ty3逆转录转座子和逆转录病毒的情况。我们的数据，以及最近对Ty3的研究，加强了3'二核苷酸切割不限于逆转录病毒整合酶的想法，并且可能是许多不同逆转录转座子的酶所共有的特征。
The tobacco retrotransposon Tnt1 can transpose through an RNA intermediate in the heterologous host Arabidopsis thaliana. We report here the identification and characterization of extrachromosomal linear and circular DNA forms of Tnt1 in this heterologous host. Our results demonstrate that Tnt1 linear intermediates possess two extra base pairs at each end compared with Tnt1's integrated forms. Prior to integration into the host genome, the two terminal nucleotides at the 3' end of these linear intermediates are removed, as in the case of the yeast Ty3 retrotransposon and of retroviruses. Our data, together with those from recent studies of Ty3, reinforce the idea that 3' dinucleotide cleavage is not restricted to retroviral integrases and is probably a feature shared by many different retrotransposons' enzymes.