Novel intracellular effects of human connective tissue growth factor expressed in Cos-7 cells
Novel intracellular effects of human connective tissue growth factor expressed in Cos-7 cells
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DOI：
10.1016/s0014-5793(00)01573-8
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发表时间：
2000-05-26
期刊：
影响因子：
3.5
通讯作者：
Takigawa, M
中科院分区：
文献类型：
--
作者：
Kubota, S;Hattori, T;Takigawa, M
为了阐明结缔组织生长因子（CTGF）的多功能性，我们通过瞬时表达研究了新生CTGF在细胞内的作用。在Cos-7细胞中，CTGF的表达导致细胞形态改变，DNA含量增加，细胞膜松散，细胞处于G2/M期。CTGF的过表达不能诱导细胞生长，而重组CTGF有效地刺激细胞外增殖。这些结果表明，细胞内CTGF可能作为一种抗有丝分裂剂，因此还应该注意到，新生的CTGF被发现聚集在中央有丝分裂机器周围。(C)2000年欧洲生物化学学会联合会。
To clarify the multiple functionality of connective tissue growth factor (CTGF), we examined the effects of nascent CTGF within the cell by transient expression. Tn Cos-7 cells, expression of human CTGF induced an altered cell morphology, It was associated with an increased cellular DNA content and loose attachment, indicating the cells mere in G2/M phase. Overexpression of CTGF did not induce cell growth, whereas recombinant CTGF efficiently stimulated the proliferation extracellularly, These results indicate that intracellular CTGF may act as an antimitotic agent, thus it should also be noted that nascent CTGF was found to accumulate around the central mitotic machinery. (C) 2000 Federation of European Biochemical Societies.