UPA-Seq-Based Search Method for Functional lncRNA Candidates
UPA-Seq-Based Search Method for Functional lncRNA Candidates
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基于 UPA-Seq 的功能性 lncRNA 候选物搜索方法
DOI：
10.1007/978-1-0716-2380-0_16
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Nakagawa Shinichi
中科院分区：
文献类型：
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作者：
Yokoi Saori;Nakagawa Shinichi
长非编码RNAs(Long Non Coding RNAs，LncRNAs)是哺乳动物转录组的重要组成部分，近年来的研究揭示了LncRNAs在多种生物过程中的重要作用。然而，已被功能验证的LncRNAs的比例很小，并且大多数LncRNAs只有序列和表达信息。在这里，我们描述了UV-苯酚水相RNA测序(uPA-seq)的过程，这是一种在整个基因组中寻找功能性lncRNA候选的方法，该方法基于功能性lncRNA通过与蛋白质结合来发挥其功能的假设。
Long noncoding RNAs (lncRNAs) constitute a large fraction of the transcriptome in mammals, and recent studies have revealed important functions of lncRNAs in a variety of biological processes. However, the fraction of lncRNAs that have been functionally validated is small, and only sequence and expression information are available for most lncRNAs. Here, we describe the procedures for UV-phenol aqueous-phase RNA sequencing (UPA-seq), a method for searching for functional lncRNA candidates among whole genomes based on the assumption that functional lncRNAs exert their functions through associations with proteins.