Regulons of global transcription factors in Corynebacterium glutamicum
Regulons of global transcription factors in Corynebacterium glutamicum
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DOI：
10.1007/s00253-015-7074-3
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发表时间：
2015
影响因子：
5
通讯作者：
Koichi Toyoda;M. Inui
中科院分区：
文献类型：
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作者：
Koichi Toyoda;M. Inui
谷氨酸棒杆菌（Corynebacterium glutamicum）是放线菌中的一种高GC含量的革兰氏阳性土壤细菌，已被用于氨基酸的工业生产，并被工程化以生产各种化合物，包括聚合物结构单元和生物燃料。自其基因组序列首次公布以来，其多功能代谢途径及其遗传组成和调控机制已被广泛研究。以往对细菌中转录因子（包括双组分系统和σ因子）的研究揭示了代谢途径和应激反应系统之间的转录调控联系，形成了复杂的转录调控网络。调控环节是基于对转录因子的了解，如其靶基因（调节子）、用于识别的DNA序列基序和控制其活性的效应分子，所有这些都是理解其生理功能的基础。基于染色质免疫沉淀（ChIP）的全基因组分析的最新进展提供了一个机会，以全面确定转录因子调节子，其直接靶基因组成，其精确的共识结合基序。调节子成分之间的共同特征可以提供线索，以确定靶向因子的效应分子。在这篇简短的综述中，我们总结了目前的知识的调节子的C。这些转录因子已经通过基于ChIP的技术进行了分析。由直接靶基因组成的调节子揭示了转录因子的新生理作用和新的调控相互作用，有助于完善和扩展转录调控网络，并为C.谷氨酸。
Corynebacterium glutamicum, a high GC content gram-positive soil bacterium in Actinobacteria, has been used for the industrial production of amino acids and engineered to produce various compounds, including polymer building blocks and biofuels. Since its genome sequence was first published, its versatile metabolic pathways and their genetic components and regulatory mechanisms have been extensively studied. Previous studies on transcriptional factors, including two-component systems and σ factors, in the bacterium have revealed transcriptional regulatory links among the metabolic pathways and those among the stress response systems, forming a complex transcriptional regulatory network. The regulatory links are based on knowledge of the transcription factors, such as their target genes (regulons), DNA sequence motifs for recognition, and effector molecules controlling their activities, all of which are fundamental for understanding their physiological functions. Recent advances in chromatin immunoprecipitation (ChIP)-based genome-wide analyses provide an opportunity to comprehensively identify the transcription factor regulon, composed of its direct target genes, and its precise consensus binding motif. A common feature among the regulon constituents may provide clues to identify an effector molecule targeting the factor. In this mini-review, we summarize the current knowledge of the regulons of theC. glutamicumtranscription factors that have been analyzed via ChIP-based technologies. The regulons consisting of direct target genes revealed new physiological roles of the transcription factors and new regulatory interactions, contributing to refinement and expansion of the transcriptional regulatory network and the development of guidelines and genetic tools for metabolic engineering ofC. glutamicum.