A high-throughput platform for detailed lipidomic analysis of a range of mouse and human tissues
A high-throughput platform for detailed lipidomic analysis of a range of mouse and human tissues
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DOI：
10.1007/s00216-020-02511-0
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发表时间：
2020-03-07
影响因子：
4.3
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中科院分区：
文献类型：
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脂质组学在生物系统的研究中越来越受到人们的关注。然而，高通量数据收集和处理仍然不是微不足道的，这使得表型评估变得困难。我们描述了一种用于测量一系列组织的脂肪分数的平台。这些技术在一组七种不同的组织(血清、脑、心脏、肾脏、脂肪、肝脏和股外侧肌)上进行了演示，这些组织来自断奶后的小鼠大坝，这些组织要么肥胖(12克脂肪质量)，要么瘦(
Lipidomics is of increasing interest in studies of biological systems. However, high-throughput data collection and processing remains non-trivial, making assessment of phenotypes difficult. We describe a platform for surveying the lipid fraction for a range of tissues. These techniques are demonstrated on a set of seven different tissues (serum, brain, heart, kidney, adipose, liver, and vastus lateralis muscle) from post-weaning mouse dams that were either obese (> 12 g fat mass) or lean (