Maturation of the Escherichia coli divisome occurs in two steps
Maturation of the Escherichia coli divisome occurs in two steps
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DOI：
10.1111/j.1365-2958.2005.04502.x
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发表时间：
2005-03-01
影响因子：
3.6
通讯作者：
den Blaauwen, T
中科院分区：
文献类型：
--
作者：
Aarsman, MEG;Piette, A;den Blaauwen, T
细胞分裂蛋白FtsZ（FtsA、ZipA、ZapA）、FtsE/X、FtsK、FtsQ、FtsL/B、FtsW、PBP 3、FtsN和AmiC以所列的相互依赖的顺序定位于大肠杆菌中的中间细胞。为了研究这是否反映了分裂体的时间依赖性成熟，通过以各种生长速率的稳态生长细胞的免疫和GFP荧光显微镜测定FtsZ、FtsQ、FtsW、PBP 3和FtsN到达其目的地的平均细胞年龄。一致的，14 - 21分钟的时间延迟，取决于生长速度，之间的Z-环的形成和中间细胞募集蛋白质下游的FtsK被发现。我们提出了一个两步模型的细菌分裂中，Z-环是参与开关从圆柱形到极性肽聚糖合成，而后来定位的细胞分裂蛋白则负责将包膜形状修改为两个新极点。
Cell division proteins FtsZ ( FtsA, ZipA, ZapA), FtsE/X, FtsK, FtsQ, FtsL/B, FtsW, PBP3, FtsN and AmiC localize at mid cell in Escherichia coli in an interdependent order as listed. To investigate whether this reflects a time dependent maturation of the divisome, the average cell age at which FtsZ, FtsQ, FtsW, PBP3 and FtsN arrive at their destination was determined by immuno- and GFP- fluorescence microscopy of steady state grown cells at a variety of growth rates. Consistently, a time delay of 14 - 21 min, depending on the growth rate, between Z-ring formation and the mid cell recruitment of proteins down stream of FtsK was found. We suggest a two-step model for bacterial division in which the Z-ring is involved in the switch from cylindrical to polar peptidoglycan synthesis, whereas the much later localizing cell division proteins are responsible for the modification of the envelope shape into that of two new poles.