RME-8 regulates trafficking of the epidermal growth factor receptor
RME-8 regulates trafficking of the epidermal growth factor receptor
复制标题
DOI：
10.1016/j.febslet.2008.02.042
复制
发表时间：
2008-03-19
期刊：
影响因子：
3.5
通讯作者：
McPherson, Peter S.
中科院分区：
文献类型：
--
作者：
Girard, Martine;McPherson, Peter S.
我们最近鉴定了受体介导的内吞作用 8 (RME-8)，一种定位于内体的 DnaJ 结构域蛋白。我们现在证明，RME-8 耗竭会导致表皮生长因子受体 (EGFR) 水平降低，而不会影响主要再循环到质膜的受体。在表面和细胞内池中均检测到 EGFR 的减少，这是由于 EGFR 降解率增加所致。有趣的是，RME-8 耗竭还会降低乳腺癌细胞系中的 EGFR 水平，其中 EGFR 家族成员 ErbB2 的过度表达已被证明可以保护 EGFR 免遭降解。这些数据表明 RME-8 在内体水平上影响 EGFR 的分类决策，并指出 RME-8 作为 ErbB2 阳性乳腺癌的潜在调控靶点。 (C) 2008 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
We recently identified receptor-mediated endocytosis 8 (RME-8), a DnaJ domain protein localized to endosomes. We now demonstrate that RME-8 depletion leads to decreased levels of epidermal growth factor receptor (EGFR) without influencing receptors that primarily recycle to the plasma membrane. Decreases in EGFR are detected at both surface and intracellular pools and result from increased rates of EGFR degradation. Interestingly, RME-8 depletion also decreases EGFR levels in breast cancer cell lines in which overexpression of the EGFR family member ErbB2 has been shown to protect EGFR from degradation. These data implicate RME-8 in sorting decisions influencing EGFR at the level of endosomes and point to RME-8 as a potential regulatory target in ErbB2-positive breast cancers. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.