MOTILITY OF RABBIT SPERMATOZOA RECOVERED FROM THE FEMALE REPRODUCTIVE-TRACT
MOTILITY OF RABBIT SPERMATOZOA RECOVERED FROM THE FEMALE REPRODUCTIVE-TRACT
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DOI：
10.1002/mrd.1120020105
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
KATZ, DF
中科院分区：
文献类型：
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作者：
COOPER, GW;OVERSTREET, JW;KATZ, DF
在单次交配后，每隔 1 分钟至 16 小时，通过相差显微镜对从阴道、子宫颈内膜、子宫和输卵管四个区域回收的兔精子的活力进行目视评估。每个样品中运动细胞的百分比取决于恢复温度，即 23°C 与 37°C，但不受观察温度的影响。输卵管下峡部的精子是对温度最敏感的群体，在 23°C 时恢复。当所有操作和观察均在 37°C 下进行时，具有前向运动能力的精子的百分比根据采样区域和交配后间隔的不同而变化。来自阴道、子宫和输卵管上部区域的细胞群通常具有高比例的具有旺盛鞭毛活性的渐进运动细胞。从子宫颈内膜和输卵管峡部下部 2 cm 恢复后，表现出进行性运动的精子较少，并且其鞭毛活动普遍减弱。后一区域鞭毛跳动频率的降低可能是限制精子在雌性管道中上升的主要因素。在交配后 6 至 16 小时从输卵管取出的群体中，仅观察到一种独特的“激活”运动模式。这种运动模式由线性渐进运动和剧烈非渐进运动的交替发作组成，可能类似于针对获能啮齿动物精子所描述的激活运动。
The motility of rabbit spermatozoa recovered from the vagina, endocervix, uterus, and four regions of the oviduct was assessed visually by phase‐contrast microscopy at intervals from one minute to 16 hours after a single mating. The percentage of motile cells in each sample was dependent on the temperature of recovery, ie, 23° vs 37°C, but was not influenced by the temperature of observation. Spermatozoa in the lower isthmus of the oviduct were the most temperature sensitive population to recovery at 23°C. When all manipulations and observations were performed at 37°C, the percentage of spermatozoa with progressive motility varied according to the region sampled and interval after mating. Populations from the vagina, uterus and upper regions of the oviduct usually had a high proportion of progressively motile cells with vigorous flagellar activity. Fewer spermatozoa showed progressive movement on recovery from the endocervix and lower 2 cm of the tubal isthmus and their flagellar activity was generally depressed. The decrease in flagellar beat frequency noted in the latter regions may be a major factor limiting sperm ascent in the female tract. A unique pattern of “activated” motility was seen exclusively in populations taken from the oviducts at 6 to 16 hours after mating. This motility pattern, consisting of alternating episodes of linear progressive and vigorous nonprogressive movement, may be analogous to the activated motility described for capacitated rodent spermatozoa.