Solid-pseudopapillary tumor of the pancreas - Immunohistochemical localization of neuroendocrine markers and CD10
Solid-pseudopapillary tumor of the pancreas - Immunohistochemical localization of neuroendocrine markers and CD10
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DOI：
10.1097/00000478-200010000-00005
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发表时间：
2000-10-01
影响因子：
5.6
通讯作者：
Okada, S
中科院分区：
文献类型：
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作者：
Notohara, K;Hamazaki, S;Okada, S
为了阐明胰腺实性假乳头状瘤 (SPT) 中的神经内分泌分化和 CD10 表达，我们对 19 例此类肿瘤进行了免疫组织化学分析，其中包括 1 例实性假乳头状癌 (SPC)、20 例胰腺神经内分泌肿瘤 (PNT)、6 例腺泡细胞癌 (ACC) 和 1 例胰腺神经内分泌肿瘤 (PNT)。 胰母细胞瘤（PB）。我们使用针对 CD56、突触素、蛋白基因产物 9.5、Go 蛋白的 α 亚基、嗜铬粒蛋白 A、CD10、胰蛋白酶、糜蛋白酶、各种细胞角蛋白 (CK)、CA19-9、波形蛋白和 α-L-抗胰蛋白酶 (AAT) 的抗血清。所有 SPT 都表现出 CD56 和 CD10 的免疫反应性，并且 15 个 SPT 集中表达除嗜铬粒蛋白 A 之外的其他神经内分泌标记物。注意到突触素阳性细胞频繁聚集。两例病例含有一个特殊的结节，其细胞形态学和免疫组织化学类似于 PNT。 1 个 SPC 中 CD10 阳性细胞很少。 PNT 呈 CD56 阳性，但强度通常较弱，其他神经内分泌标记物（包括嗜铬粒蛋白 A）的染色呈弥漫性阳性。在 5 个 PNT 中检测到 CD10，大部分呈焦点模式。 Pan-CK、CK8、CK18 和 CK19 在 PNT 中比 SPT 中更常见。波形蛋白和 AAT 也经常在 PNT 中被识别，但并不是 SPT 所特有的。除一例被指定为混合性腺泡内分泌癌的病例外，ACC 均为 CD56 阴性。 PB 在肿瘤巢周围 CD56 呈局灶性阳性。 4 个 ACC 和 1 个 PB 表现出局部 CD10 反应性。本研究证明了SPT独特的免疫组织化学特征。我们的结果还表明，SPT 至少在局部表现出神经内分泌分化，并且这些神经内分泌标记物和 CD10 在诊断上是有用的。
To clarify the neuroendocrine differentiation and CD10 expression in solid-pseudopapillary tumors (SPTs) of the pancreas, we performed immunohistochemical analysis in 19 such tumors, including one solid-pseudopapillary carcinoma (SPC), along with 20 pancreatic neuroendocrine tumors (PNTs), six acinar cell carcinomas (ACCs), and one pancreatoblastoma (PB). We used antisera directed against CD56, synaptophysin, protein gene product 9.5, the alpha-subunit of Go protein, chromogranin A, CD10, trypsin, chymotrypsin, various cytokeratins (CKs), CA19-9, vimentin, and alpha-l-antitrypsin (AAT). All SPTs exhibited immunoreactivity for CD56 and CD10, and 15 expressed other neuroendocrine markers focally with the exception of chromogranin A. Frequent clustering of synaptophysin-positive cells was noted. Two cases contained a peculiar nodule that cytomorphologically and immunohistochemically resembled PNT. CD10-positive cells were scarce in one SPC. PNTs were CD56-positive, but often with faint intensity, and staining for other neuroendocrine markers, including chromogranin A, was diffusely positive. CD10 was detected, mostly in a focal pattern, in five PNTs. Pan-CK, CK8, CK18, and CK19 were more frequently demonstrated in PNT than SPT. Vimentin and AAT were often identified in PNT as well and were not specific for SPT. ACCs were CD56-negative, with the exception of one case designated as a mixed acinar-endocrine carcinoma. PB was focally positive for CD56 at the periphery of the tumor nests. Four ACCs and one PB exhibited focal CD10 reactivity. This study demonstrated the unique immunohistochemical features of SPT. Our results also suggest that SPT exhibits, at least focally, neuroendocrine differentiation, and that these neuroendocrine markers and CD10 are diagnostically useful.