Dissection of the lymphokine-activated killer phenomenon. Relative contribution of peripheral blood natural killer cells and T lymphocytes to cytolysis.
Dissection of the lymphokine-activated killer phenomenon. Relative contribution of peripheral blood natural killer cells and T lymphocytes to cytolysis.
复制标题
解剖淋巴细胞激活的杀手现象。外周血天然杀伤细胞和T淋巴细胞对胞溶性的相对贡献。
DOI：
10.1084/jem.164.3.814
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发表时间：
1986-09-01
期刊：
影响因子：
--
通讯作者：
Lanier LL
中科院分区：
文献类型：
--
作者：
Phillips JH;Lanier LL
人外周血淋巴细胞在IL-2中的体外培养导致细胞毒性细胞的产生，所述细胞毒性细胞可以裂解新鲜的和培养的实体瘤细胞以及造血肿瘤细胞系，而无需刻意免疫或MHC限制。这被称为淋巴因子激活的杀伤细胞（LAK）现象。在这里，我们表明，这种活性的大部分是由表达Leu-19（NKH-1）抗原，但不表达CD 3的NK细胞介导的。该效应子群体的前体也表达表型CD 3-、Leu-19+。外周血CD 3 + T淋巴细胞对LAK现象的贡献很小，尽管低水平的非MHC限制性细胞毒性对造血肿瘤细胞靶点介导的CD 3 + T淋巴细胞亚群，共表达Leu-19抗原。这些研究清楚地表明，LAK现象不是由独特的LAK细胞介导的，而是主要由IL-2激活的外周血NK细胞介导的。
In vitro culture of human peripheral blood lymphocytes in IL-2 results in the generation of cytotoxic cells that can lyse fresh and cultured solid tumor cells, as well as hematopoietic tumor cell lines, without deliberate immunization or MHC restriction. This has been referred to as the lymphokine activated killer (LAK) phenomenon. Here, we show that the majority of this activity is mediated by NK cells that express the Leu-19 (NKH-1) antigen, but do not express CD3. The precursor of this effector population also expressed the phenotype CD3-, Leu-19+. Peripheral blood CD3+ T lymphocytes contributed little to the LAK phenomenon, although low levels of non-MHC restricted cytotoxicity against hematopoietic tumor cell targets were mediated by a subset of CD3+ T lymphocytes that coexpressed the Leu-19 antigen. These studies clearly indicated that the LAK phenomenon is not mediated by a unique LAK cell, but is mediated mainly by IL-2-activated peripheral blood NK cells.