Post-transcriptional modification of RNAs by artificial Box H/ACA and Box C/D RNPs.
Post-transcriptional modification of RNAs by artificial Box H/ACA and Box C/D RNPs.
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DOI：
10.1007/978-1-61779-018-8_14
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
Yu, Yi-Tao
中科院分区：
文献类型：
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作者：
Huang, Chao;Karijolich, John;Yu, Yi-Tao
RNA引导的RNA 2′-O-甲基化和假尿苷化是天然发生的过程，其中引导RNA特异性地指导真核细胞核中rRNA或剪接体snRNA的修饰。修饰可以深刻地改变RNA的性质，从而影响RNA对其参与的细胞过程的贡献。最近，已经表明，通过表达人工向导RNA（源自天然存在的向导RNA），修饰也可以特异性地引入到其他RNA中，从而提供了在体内研究RNA的机会。在这里，我们提出了构建指导RNA和操纵核中的RNA修饰的策略。
RNA-guided RNA 2′-O-methylation and pseudouridylation are naturally occurring processes, in which guide RNAs specifically direct modifications to rRNAs or spliceosomal snRNAs in the nucleus of eukaryotic cells. Modifications can profoundly alter the properties of an RNA, thus influencing the contributions of the RNA to the cellular process in which it participates. Recently, it has been shown that, by expressing artificial guide RNAs (derived from naturally occurring guide RNAs), modifications can also be specifically introduced into other RNAs, thus offering an opportunity to study RNAs in vivo. Here, we present strategies for constructing guide RNAs and manipulating RNA modifications in the nucleus.