Cloning of a rice cDNA encoding a transcription factor homologous to barley GAMyb

Cloning of a rice cDNA encoding a transcription factor homologous to barley GAMyb
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DOI:
10.1093/oxfordjournals.pcp.a029175
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发表时间:
1997-03-01
影响因子:
4.9
通讯作者:
Jacobsen, JV
Jacobsen, JV
中科院分区:
生物学2区
文献类型:
--
作者:
Gubler, F;Watts, RJ;Jacobsen, JV

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从水稻胚乳cDNA文库中分离到一个与大麦Myb类转录因子HvGAMSyb同源的cDNA克隆OsGAmyB。该克隆被用来证明OsCAmyb基因在糊粉细胞中的表达受到赤霉酸的刺激,瞬时表达分析表明该基因产物反式激活了α-淀粉酶基因的启动子。
A cDNA clone, OsGAmyB, which encodes a homologue to the barley Myb-like transcription factor, HvGAMSyb, was isolated from a rice endosperm cDNA library. The clone was used to show that expression of the OsCAmyb gene in aleurone cells was stimulated by gibberellic acid and the gene product was shown to transactivate an alpha-amylase gene promoter in transient expression analyses.