A real-time colorimetric assay for label-free detection of microRNAs down to sub-femtomolar levels.
A real-time colorimetric assay for label-free detection of microRNAs down to sub-femtomolar levels.
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DOI：
10.1039/c3cc41565a
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发表时间：
2013-05
影响因子：
4.9
通讯作者：
W. Shen;Huimin Deng;Yuqian Ren;Zhiqiang Gao
中科院分区：
文献类型：
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作者：
W. Shen;Huimin Deng;Yuqian Ren;Zhiqiang Gao
提出了一种利用金纳米颗粒网络和微RNA特异性核酸酶（DSN）进行无标记检测的实时比色分析。优异的灵敏度和选择性是通过DSN的参与和累积信号放大过程来实现的。
A real-time colorimetric assay utilizing gold nanoparticle networks and a duplex-specific nuclease (DSN) for label-free detection of microRNAs is proposed. Excellent sensitivity and selectivity were accomplished through the engagement of the DSN and a cumulative signal amplification process.