Evidence for a shared nuclear pore complex architecture that is conserved from the last common eukaryotic ancestor.
Evidence for a shared nuclear pore complex architecture that is conserved from the last common eukaryotic ancestor.
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DOI：
10.1074/mcp.m900038-mcp200
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发表时间：
2009-09
期刊：
影响因子：
--
通讯作者：
Chait BT
中科院分区：
文献类型：
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作者：
DeGrasse JA;DuBois KN;Devos D;Siegel TN;Sali A;Field MC;Rout MP;Chait BT
核孔复合体(NPC)是嵌入核膜中的一种大分子组合体，它介导了核质之间的双向物质交换。我们最近对酵母NPC的研究揭示了其结构中的一种简单的模块化，并暗示了NPC和前体原生生物中的囊泡涂层复合体的共同进化起源。然而，详细的组成和结构信息目前仅适用于脊椎动物和酵母NPC，它们在进化上密切相关。因此，我们对鼻咽癌组成的理解在完全进化的背景下是稀疏的。此外，尽管鼻咽癌的性质无处不在，但在遥远的分类群中进行的序列搜索发现的鼻咽癌成分出奇地少，这表明鼻咽癌的大部分可能不是保守的。因此，为了对鼻咽癌的起源和进化有一个更广阔的视角，我们对一个分化的真核生物(布氏锥虫)的含鼻咽癌部分进行了蛋白质组学分析，并获得了其核孔蛋白的全面清单。值得注意的是，锥虫核孔蛋白与后生动物和酵母菌具有明显的相同的折叠类型、结构域组织、组成和模块化。总体而言，这些数据提供了确凿的证据，表明鼻咽癌的大部分结构确实在整个真核生物中是保守的，并且已经在最后一个共同的真核祖先中建立起来。这些发现有力地支持了这样的假设，即神经干细胞与囊泡包衣复合体有着共同的祖先，并且两者都是在真核生物进化的非常早期就建立起来的。
The nuclear pore complex (NPC) is a macromolecular assembly embedded within the nuclear envelope that mediates bidirectional exchange of material between the nucleus and cytoplasm. Our recent work on the yeast NPC has revealed a simple modularity in its architecture and suggested a common evolutionary origin of the NPC and vesicle coating complexes in a progenitor protocoatomer. However, detailed compositional and structural information is currently only available for vertebrate and yeast NPCs, which are evolutionarily closely related. Hence our understanding of NPC composition in a full evolutionary context is sparse. Moreover despite the ubiquitous nature of the NPC, sequence searches in distant taxa have identified surprisingly few NPC components, suggesting that much of the NPC may not be conserved. Thus, to gain a broad perspective on the origins and evolution of the NPC, we performed proteomics analyses of NPC-containing fractions from a divergent eukaryote (Trypanosoma brucei) and obtained a comprehensive inventory of its nucleoporins. Strikingly trypanosome nucleoporins clearly share with metazoa and yeast their fold type, domain organization, composition, and modularity. Overall these data provide conclusive evidence that the majority of NPC architecture is indeed conserved throughout the Eukaryota and was already established in the last common eukaryotic ancestor. These findings strongly support the hypothesis that NPCs share a common ancestry with vesicle coating complexes and that both were established very early in eukaryotic evolution.