EVOLUTION OF CODON USAGE PATTERNS - THE EXTENT AND NATURE OF DIVERGENCE BETWEEN CANDIDA-ALBICANS AND SACCHAROMYCES-CEREVISIAE
EVOLUTION OF CODON USAGE PATTERNS - THE EXTENT AND NATURE OF DIVERGENCE BETWEEN CANDIDA-ALBICANS AND SACCHAROMYCES-CEREVISIAE
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DOI：
10.1093/nar/20.20.5289
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发表时间：
1992-10-25
影响因子：
14.9
通讯作者：
SHARP, PM
中科院分区：
文献类型：
--
作者：
LLOYD, AT;SHARP, PM
使用多变量统计分析法分析了致病性酵母菌白色念珠菌28个基因的密码子使用情况。一个主要的趋势基因，相关的基因表达水平，被确定。我们集中在白色念珠菌和密切相关的酵母酿酒酵母之间的分歧的程度和性质。最近有人提出，在这两个物种中优选密码子的子集之间存在显著差异[Brown等人（1991）Nucleic Acids Res.19，4293]。总的来说，白色念珠菌的基因更富含A + T，反映了该物种较低的基因组G + C含量，推测是由不同的突变偏倚模式引起的。然而，在这两个物种中，高表达的基因优先使用相同的“最佳”密码子子集。一个建议，NCG密码子的频率低，在两种酵母菌的结果选择对密码子的存在，是潜在的高度可变的折扣。C.密码子使用与其他单细胞物种一样，白色念珠菌的突变可以解释为突变偏好和翻译选择过程之间平衡的结果。在两个相关的念珠菌属物种，麦芽糖念珠菌和C. Tropicalis，简要讨论。
Codon usage in a sample of 28 genes from the pathogenic yeast Candida albicans has been analysed using multivariate statistical analysis. A major trend among genes, correlated with gene expression level, was identified. We have focussed on the extent and nature of divergence between C.albicans and the closely related yeast Saccharomyces cerevisiae. It was recently suggested that significant differences exist between the subsets of preferred codons in these two species [Brown et al. (1991) Nucleic Acids Res. 19, 4293]. Overall, the genes of C.albicans are more A + T-rich, reflecting the lower genomic G + C content of that species, and presumably resulting from a different pattern of mutational bias. However, in both species highly expressed genes preferentially use the same subset of 'optimal' codons. A suggestion that the low frequency of NCG codons in both yeast species results from selection against the presence of codons that are potentially highly mutable is discounted. Codon usage in C. albicans, as in other unicellular species, can be interpreted as the result of a balance between the processes of mutational bias and translational selection. Codon usage in two related Candida species, C.maltosa and C. tropicalis, is briefly discussed.