Avian erythroblastosis virus: transformation-specific sequences form a contiguous segment of 3.25 kb located in the middle of the 6-kb genome.
Avian erythroblastosis virus: transformation-specific sequences form a contiguous segment of 3.25 kb located in the middle of the 6-kb genome.
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禽成红细胞增多症病毒：转化特异性序列形成位于 6 kb 基因组中部的 3.25 kb 连续片段。
DOI：
10.1016/0042-6822(79)90347-7
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发表时间：
1979
期刊：
影响因子：
3.7
通讯作者：
P. Vogt
中科院分区：
文献类型：
--
作者：
M. Lai;S. S. Hu;P. Vogt
禽成红细胞增多症病毒（AEV）ES-4株转化鸡胚成纤维细胞的几个病灶被发现产生含有超过辅助病毒的RNA的复制缺陷型AEV的RNA的病毒子代。经甲基汞-琼脂糖凝胶电泳和电镜观察，AEV RNA的大小为28 S或6 kb。通过RNA-DNA杂交，约40%至45%的这种RNA与其他鸡白血病和肉瘤病毒的RNA同源;基因组的其余部分是AEV特异性的。这些AEV特异性序列可能包含负责转化的遗传信息，形成3.25 kb的连续片段，通过异源双链体作图定位在6 kb基因组的中心，位于3′端1.06 kb和5′端1.64 kb的两个片段之间，这两个片段与禽肉瘤病毒基因组同源。根据在基因组的5′端显示AEV和禽肉瘤病毒之间同源性的区域的长度和AEV特异性75 K蛋白的已知序列组成（Haymanet al.，1979），推测禽肿瘤病毒gag蛋白p19的N端起始点位于距基因组5′端约1.0kb处。非生产AEV转化的鸡胚成纤维细胞也被分离。感染性AEV只能用鸡白血病病毒从这些细胞中拯救出来;无关的禽逆转录病毒无效，可能是因为AEV除了env外，还需要gag和polgenes的互补。
Several foci of chicken embryo fibroblasts transformed by avian erythroblastosis virus (AEV) strain ES-4 were found to produce virus progeny containing the RNA of the replication-defective AEV in excess over the RNA of the helper virus. The size of AEV RNA was determined by methylmercury-agarose gel electrophoresis and electron microscopy to be 28 S or 6 kb. About 40 to 45% of this RNA is homologous by RNA-DNA hybridization to the RNA of other chicken leukosis and sarcoma viruses; the rest of the genome is AEV specific. These AEV specific sequences, which presumably contain the genetic information responsible for transformation form a contiguous stretch of 3.25 kb, located by heteroduplex mapping in the center of the 6 kb genome between two segments of 1.06 kb at the 3′ end and 1.64 kb at the 5′ end which are homologous to the genome of avian sarcoma virus. From the length of the region showing homology between AEV and avian sarcoma virus at the 5′ end of the genome and from the known sequence composition of the AEV-specific 75K protein (Haymanet al., 1979), it can be deduced that the initiation point for the N terminus of thegagprotein p19 is located about 1.0 kb from the 5′ end of the genome in avian oncoviruses. Nonproducing AEV-transformed chicken embryo fibroblasts were also isolated. Infectious AEV could be rescued from these cells only with chicken leukosis viruses; unrelated avian retroviruses were ineffective, probably because AEV requires complementation in thegagandpolgenes, in addition toenv.