Isolation and characterization of microsatellite markers in Japanese pear (Pyrus pyrifolia Nakai)
Isolation and characterization of microsatellite markers in Japanese pear (Pyrus pyrifolia Nakai)
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DOI：
10.1111/j.1471-8286.2006.01612.x
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发表时间：
2007-05-01
期刊：
影响因子：
--
通讯作者：
Evans, Kate
中科院分区：
文献类型：
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作者：
Inoue, Eiichi;Matsuki, Yumi;Evans, Kate
利用选择杂交技术，从日本梨基因组文库中筛选出14个微卫星标记。它们的特点是使用17个日本梨品种。期望杂合度和观察杂合度分别为0.21 ~ 0.74和0 ~ 0.88。每个位点检测到2至11个等位基因，其中IPPN 09和IPPN 15被判定为扩增多个位点。IPPN 17是信息量最大的位点，同一性概率最低（0.19）。这些引物具有较高的种间和属间通用性。
Fourteen microsatellite markers were developed from an enriched genomic library of Japanese pear (Pyrus pyrifolia) by selective hybridization. They were characterized using 17 Japanese pear cultivars. The expected heterozygosity and observed heterozygosity ranged from 0.21 to 0.74 and from 0 to 0.88, respectively. Two to 11 alleles were detected per locus, with IPPN09 and IPPN15 judged to amplify multiple loci. IPPN17 was the most informative locus with the lowest probability of identity (0.19). These primers exhibited a high cross-species transferability between species and genera.