iTRAQ-based proteomics analysis of the therapeutic effects of combined anticancer bioactive peptides and oxaliplatin on gastric cancer cells
iTRAQ-based proteomics analysis of the therapeutic effects of combined anticancer bioactive peptides and oxaliplatin on gastric cancer cells
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DOI：
10.3892/or.2019.7406
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发表时间：
2020-01-01
期刊：
影响因子：
4.2
通讯作者：
Su, Xiulan
中科院分区：
文献类型：
--
作者：
Xu, Yanan;Li, Xian;Su, Xiulan
联合化疗治疗胃癌可能比单独化疗更有效。既往研究表明，抗癌生物活性肽（ACBP）联合奥沙利铂（OXA）可显著抑制胃癌细胞系MKN-45的生长，促进MKN-45细胞的凋亡，使MKN-45细胞周期不可逆地阻滞在G2/M期。本研究采用等压标记相对绝对定量（iTRAQ）定量蛋白质组学技术，测定ACBP-OXA处理对MKN-45细胞蛋白质组学谱的影响。值得注意的是，共检测到6210个蛋白质。表达变化（上调或下调）为> - 1.2倍的蛋白和P
The combination of chemotherapeutic modalities may be more effective in treating gastric cancer compared with any modality alone. Previous studies have demonstrated that the combination of anticancer bioactive peptides (ACBP) and oxaliplatin (OXA) significantly inhibited the growth of the gastric cancer cell line MKN-45, promoted the apoptosis of MKN-45 cells, and caused an irreversible arrest of the MKN-45 cell cycle in the G2/M phase. In the present study, an isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomics technique was used to determine the effect of ACBP-OXA treatment on the proteomics profile of MKN-45 cells. Notably, a total of 6,210 proteins were detected. Proteins with a >1.2-fold change in expression (either up- or downregulation) and P