Myc requires distinct E2F activities to induce S phase and apoptosis
Myc requires distinct E2F activities to induce S phase and apoptosis
复制标题
DOI：
10.1016/s1097-2765(01)00275-1
复制
发表时间：
2001-07-01
期刊：
影响因子：
16
通讯作者：
Nevins, JR
中科院分区：
文献类型：
--
作者：
Leone, G;Sears, R;Nevins, JR
先前的研究表明，Myc转录因子可诱导E2F1、E2F2和E2F3基因的转录。通过删除E2F基因的原代小鼠胚胎成纤维细胞，我们发现myc诱导的S期和凋亡需要不同的E2F活性。在缺乏E2F2或E2F3的情况下，Myc诱导S期的能力受损，但没有E2F1或E2F4。相比之下，在E2F1基因缺失的细胞中，Myc诱导细胞凋亡的能力明显降低，而E2F2或E2F3基因缺失的细胞则没有。根据这些数据，我们提出特异性E2F活性的诱导是Myc通路中控制细胞增殖和细胞命运决定的重要组成部分。
Previous work has shown that the Myc transcription factor induces transcription of the E2F1, E2F2, and E2F3 genes. Using primary mouse embryo fibroblasts deleted for individual E2F genes, we now show that Myc-induced S phase and apoptosis requires distinct E2F activities. The ability of Myc to induce S phase is impaired in the absence of either E2F2 or E2F3 but not E2F1 or E2F4. In contrast, the ability of Myc to induce apoptosis is markedly reduced in cells deleted for E2F1 but not E2F2 or E2F3. From this data, we propose that the induction of specific E2F activities is an essential component in the Myc pathways that control cell proliferation and cell fate decisions.