In Vitro Toxicity of Silver Nanoparticles at Noncytotoxic Doses to HepG2 Human Hepatoma Cells
In Vitro Toxicity of Silver Nanoparticles at Noncytotoxic Doses to HepG2 Human Hepatoma Cells
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DOI：
10.1021/es900754q
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发表时间：
2009-08-01
影响因子：
11.4
通讯作者：
Okabe, Satoshi
中科院分区：
文献类型：
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作者：
Kawata, Koji;Osawa, Masato;Okabe, Satoshi
虽然已有报道银纳米颗粒（Ag-NPs）对多种培养物具有强烈的急性毒性作用，但在非细胞毒性剂量下的毒性作用尚不清楚。因此，我们在体外评价剂量在人肝癌细胞系HepG 2中的毒性，基于细胞活力测定、微核试验和DNA微阵列。我们还使用聚苯乙烯纳米颗粒（PS-NPs）和银碳酸盐（Ag 2CO 3）作为测试材料，以比较关于不同的原始化学组成和形式的银的毒性。细胞活力测定表明，Ag-NPs在低剂量（1.0 mg/L）下加速细胞增殖和异常细胞形态，显示细胞收缩和获得不规则形状。此外，仅暴露使双核细胞的微核形成频率增加至47.9 +/- 3.2%，表明其对染色体的损伤似乎比PS-NP和离子Ag+强得多。半胱氨酸是一种强离子型Ag+，它只能部分抑制Ag-NPs介导的微核形成，并改变基因表达，表明来源于Ag-NPs的离子型Ag+不能完全解释这些生物学作用。基于这些讨论，得出结论：“纳米级Ag颗粒”和“离子型Ag+”都有助于Ag-NPs的毒性作用。
Although it has been reported that silver nanoparticles (Ag-NPs) have strong acute toxic effects to various cultured the toxic effects at noncytotoxic doses are still unknown. We, therefore, evaluated in vitro toxicity of doses in human hepatoma cell line, HepG2, based on cell viability assay, micronucleus test, and DNA microarray We also used polystyrene nanoparticles (PS-NPs) and silver carbonate (Ag2CO3) as test materials to compare the toxic with respect to different raw chemical composition and form of silver. The cell viability assay demonstrated that Ag-NPs accelerated cell proliferation at low doses ( 1.0 mg/L) and abnormal cellular morphology, displaying cellular shrinkage and acquisition of an irregular shape. In addition, only exposure increased the frequency of micronucleus formation up to 47.9 +/- 3.2% of binucleated cells, suggesting that appear to cause much stronger damages to chromosome than PS-NPs and ionic Ag+. Cysteine, a strong ionic Ag+ only partially abolished the formation of micronuclei mediated by Ag-NPs and changed the gene expression, indicating that ionic Ag+ derived from Ag-NPs could not fully explain these biological actions, Based on these discussions, it is concluded that both "nanosized particle of Ag" as well as "ionic Ag+" contribute to the toxic effects of Ag-NPs.