Mutations in the acetolactate synthase genes of sulfonylurea-resistant biotypes of Lindernia spp.
Mutations in the acetolactate synthase genes of sulfonylurea-resistant biotypes of Lindernia spp.
复制标题
林德尼亚属磺酰脲类抗性生物型的乙酰乳酸合酶基因突变。
DOI：
10.1046/j.1445-6664.2001.00054.x
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发表时间：
2002
影响因子：
1.4
通讯作者：
Hiroaki Watanabe
中科院分区：
文献类型：
--
作者：
A. Uchino;Hiroaki Watanabe
乙酰乳酸合成酶（ALS）是支链氨基酸生物合成途径中的关键酶。ALS基因的突变导致结构域A中脯氨酸位置的氨基酸取代，使得ALS对磺酰脲类除草剂不太敏感，磺酰脲类除草剂是ALS抑制性除草剂。我们从Lindernia属的4种植物中克隆了ALS基因的部分序列。杜比亚湾杜比亚湖杜比亚湾梅杰湖micrantha和L.在稻田中发现了对磺酰脲类具有抗性的生物型。在每株Lindernia植物中，将克隆分为两组：Als1和Als2。克隆的测序和推导的氨基酸序列与先前报道的其他物种的ALS的比对表明，克隆的区域包含在Als1和Als2的内含子。Als1结构域A的脯氨酸在Lindernia属植物的所有抗性生物型中均被丙氨酸、丝氨酸或谷氨酰胺取代，而在Lindernia属植物的所有感病生物型中均保持保守。Als1编码的ALS中的这种氨基酸替换参与了4种Lindernia植物ALS对磺酰脲类药物的抗性机制。
Acetolactate synthase (ALS) is a key enzyme in the biosynthetic pathway of branched-chain amino acids. A mutation of the ALS gene causing amino acid substitution at the position of proline in Domain A makes ALS less sensitive to sulfonylureas, which are ALS-inhibiting herbicides. We cloned partial ALS genes from four Lindernia plants, L. dubia var. dubia, L. dubia var. major, L. micrantha and L. procumbens, for which biotypes resistant to sulfonylureas have been found in paddy fields. The clones were classified into two groups in each Lindernia plant: Als1 and Als2. Sequencing of the clones and alignment of deduced amino acid sequences with previously reported ALS of other species suggested that the cloned region contains an intron in both Als1 and Als2. Comparison of Als1 between resistant and susceptible biotypes showed that the proline of Domain A was replaced by alanine, serine or glutamine in all resistant biotypes of Lindernia plants, while it was conserved in all susceptible biotypes. This amino acid substitution in ALS encoded by Als1 is involved in the resistant mechanism of ALS to sulfonylurea in the four Lindernia plants.