Sex differences in gut microbiota in patients with major depressive disorder.
Sex differences in gut microbiota in patients with major depressive disorder.
复制标题
重度抑郁症患者肠道微生物群的性别差异
DOI：
10.2147/ndt.s159322
复制
发表时间：
2018
影响因子：
3.2
通讯作者：
Xie P
中科院分区：
文献类型：
--
作者：
Chen JJ;Zheng P;Liu YY;Zhong XG;Wang HY;Guo YJ;Xie P
目的本课题组前期研究发现，肠道菌群失调可能在重性抑郁症（MDD）的发病中起一定作用。本研究的目的是调查MDD患者的肠道菌群是否存在性别差异。患者和方法首次发作的药物初治MDD患者和健康对照。分析从纳入受试者的粪便样本中提取的16S rRNA基因序列。主坐标分析和偏最小二乘判别分析用于评估是否存在性别特异性肠道菌群。采用随机森林算法对微分运算分类单元进行识别。线性判别分析效应量被进一步用于识别导致MDD患者和健康对照组之间差异的主要性别特异性抑郁症类型。结果共鉴定出57个和74个区分MDD患者和健康人的差异操作分类单位。与健康对照组相比，女性和男性MDD患者中放线菌数量增加，拟杆菌数量减少。女性和男性MDD患者中丰度差异最大的细菌分类群分别属于放线菌门和拟杆菌门。同时，女性和男性MDD患者存在不同的优势抑郁类型。结论MDD患者肠道菌群存在性别差异。放线菌和拟杆菌作为性别特异性生物标志物诊断MDD的适用性有待进一步探讨。
Objective Our previous studies found that disturbances in gut microbiota might have a causative role in the onset of major depressive disorder (MDD). The aim of this study was to investigate whether there were sex differences in gut microbiota in patients with MDD. Patients and methods First-episode drug-naïve MDD patients and healthy controls were included. 16S rRNA gene sequences extracted from the fecal samples of the included subjects were analyzed. Principal-coordinate analysis and partial least squares-discriminant analysis were used to assess whether there were sex-specific gut microbiota. A random forest algorithm was used to identify the differential operational taxonomic units. Linear discriminant-analysis effect size was further used to identify the dominant sex-specific phylotypes responsible for the differences between MDD patients and healthy controls. Results In total, 57 and 74 differential operational taxonomic units responsible for separating female and male MDD patients from their healthy counterparts were identified. Compared with their healthy counterparts, increased Actinobacteria and decreased Bacteroidetes levels were found in female and male MDD patients, respectively. The most differentially abundant bacterial taxa in female and male MDD patients belonged to phyla Actinobacteria and Bacteroidia, respectively. Meanwhile, female and male MDD patients had different dominant phylotypes. Conclusion These results demonstrated that there were sex differences in gut microbiota in patients with MDD. The suitability of Actinobacteria and Bacteroidia as the sex-specific biomarkers for diagnosing MDD should be further explored.