Female Rats Demonstrate Improved Locomotor Recovery and Greater Preservation of White and Gray Matter after Traumatic Spinal Cord Injury Compared to Males
Female Rats Demonstrate Improved Locomotor Recovery and Greater Preservation of White and Gray Matter after Traumatic Spinal Cord Injury Compared to Males
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DOI：
10.1089/neu.2014.3702
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发表时间：
2015-08-01
影响因子：
4.2
通讯作者：
Pearse, Damien D.
中科院分区：
文献类型：
--
作者：
Datto, Jeffrey P.;Bastidas, Johana C.;Pearse, Damien D.
脊髓损伤（SCI）后恢复的性别相关差异的可能性仍然是一个有争议的话题。目前的实验动物研究缺乏大规模的测试组，以明确确定是否存在显着差异。在精确的、临床相关的脊髓挫伤模型后评估性别之间的运动恢复差异可以为SCI后结局中可能的性别相关优势提供有价值的见解。在目前的研究中，我们假设，通过采用更大的样本量在一个可重复的挫伤SCI范例，微妙的性别之间的运动恢复的差异，如果它们存在，将通过广泛的行为测试来阐明。在大鼠胸部（T8）挫伤性SCI后13周的功能评估中，Basso、Beattie和Bresnahan评分以及CatWalk后肢摆动、支撑四个和单站分析存在性别差异。早在SCI后4周，运动表现就有显著差异。体视学组织体积分析确定，女性，比男性，也表现出更大的体积保存的灰色和白色物质内受损的脊髓节段，以及更多的备用腹侧白色物质区域的病变中心。有利于雌性的体视学组织分析差异与终点时观察到的雌性大鼠更大的功能改善直接相关。
The possibility of a gender-related difference in recovery after spinal cord injury (SCI) remains a controversial subject. Current empirical animal research lacks sizable test groups to definitively determine whether significant differences exist. Evaluating locomotor recovery variances between sexes following a precise, clinically relevant spinal cord contusion model can provide valuable insight into a possible gender-related advantage in outcome post-SCI. In the current study, we hypothesized that by employing larger sample sizes in a reproducible contusive SCI paradigm, subtle distinctions in locomotor recovery between sexes, if they exist, would be elucidated through a broad range of behavioral tests. During 13 weeks of functional assessment after a thoracic (T8) contusive SCI in rat, significant differences owing to gender existed for the Basso, Beattie, and Bresnahan score and CatWalk hindlimb swing, support four, and single stance analyses. Significant differences in locomotor performance were noticeable as early as 4 weeks post-SCI. Stereological tissue-volume analysis determined that females, more so than males, also exhibited greater volumes of preserved gray and white matter within the injured cord segment as well as more spared ventral white matter area at the center of the lesion. The stereological tissue analysis differences favoring females directly correlated with the female rats' greater functional improvement observed at endpoint.