THE CORRELATION AVERAGING OF A REGULARLY ARRANGED BACTERIAL-CELL ENVELOPE PROTEIN
THE CORRELATION AVERAGING OF A REGULARLY ARRANGED BACTERIAL-CELL ENVELOPE PROTEIN
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DOI：
10.1111/j.1365-2818.1982.tb00405.x
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发表时间：
1982-01-01
影响因子：
2
通讯作者：
BAUMEISTER, W
中科院分区：
文献类型：
--
作者：
SAXTON, WO;BAUMEISTER, W
自适应的相关平均方法的描述，它允许可靠的和几乎完全自动的图像平均的情况下，近周期性的结构，尽管存在大量的晶体缺陷;用于评估分辨率和对称性，而不依赖于结晶度的方法也进行了讨论。EM的负染色和旋转阴影制剂的HPI（六角形包装的中间）层蛋白质从耐辐射微球菌的细胞被膜的平均使用的方法，和投影结构被描述为约1.9 nm的分辨率。
An adaptation of the correlation averaging method is described which allows reliable and almost fully automatic image averaging in the case of near-periodic structures notwithstanding the presence of substantial crystal imperfections; methods for assessing resolution and symmetry without reliance on crystallinity are also discussed. EM of negatively stained and rotary shadowed preparations of the HPI (hexagonally packed intermediate)-layer protein from the cell envelope of Micrococcus radiodurans were averaged using the method, and the projected structure is described to a resolution of about 1.9 nm.