Determination of pyrethroid residues in agricultural products by an enzyme-linked immunosorbent assay
Determination of pyrethroid residues in agricultural products by an enzyme-linked immunosorbent assay
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DOI：
10.1021/jf049438z
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发表时间：
2004-09-08
影响因子：
6.1
通讯作者：
Hammock, BD
中科院分区：
文献类型：
--
作者：
Park, EK;Kim, JH;Hammock, BD
建立了检测农产品中氯氰菊酯和氯氰菊酯的竞争酶联免疫吸附分析方法。通过直接稀释萃取物，将基质干扰降至最低。不需要进一步的清理工作。当使用含40%甲醇的磷酸盐缓冲盐水作为稀释剂时，白酒对氯氰菊酯的稀释倍数为1：10，红葡萄酒、白葡萄酒、水果和蔬菜对氯氰菊酯的稀释倍数为1：200，基质效应最小。观察到加标水平的良好恢复。白葡萄酒中氯氰菊酯和红葡萄酒中氯氰菊酯的平均回收率分别为99.7%、74%和78%。苹果、香蕉、黄瓜、生菜、洋葱和桃子的平均回收率分别为99.2%、105%、70.2%、97.5%、94.4%和89.4%。用气相色谱-质谱法对添加氯氰菊酯的生菜和桃子样品进行了验证，获得了良好的回收率和相关性。
To determine cypermethrin and permethrin in agricultural products, a competitive enzyme-linked immunosorbent assay (ELISA) method was employed. The matrix interferences were minimized by direct dilution of the extracts. No further cleanup was needed. A minimum matrix effect with a 1:10 dilution of white wine for cypermethrin and a 1:200 dilution of red and white wines, fruits, and vegetables for permethrin was found when phosphate-buffered saline containing 40% methanol was employed as the diluent. Good recoveries of spiked levels were observed. The mean percentage recoveries of cypermethrin spiked in white wine and permethrin spiked in red and white wines were 99.7, 74, and 78%, respectively. The mean percentage recoveries of permethrin spiked in apple, banana, cucumber, lettuce, onion, and peach were 99.2, 105, 70.2, 97.5, 94.4, and 89.4%, respectively. Validation of the ELISA method with permethrin-spiked lettuce and peach was carried out using gas chromatography with mass spectrometry, resulting in a good recovery and correlation.