Regulation of mammalian totipotency: a molecular perspective from in vivo and in vitro studies.
Regulation of mammalian totipotency: a molecular perspective from in vivo and in vitro studies.
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DOI：
10.1016/j.gde.2023.102083
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发表时间：
2023-07
影响因子：
4
通讯作者：
Tsunetoshi Nakatani;M. Torres-Padilla
中科院分区：
文献类型：
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作者：
Tsunetoshi Nakatani;M. Torres-Padilla
细胞模型可以概括胚胎基因组激活的某些方面。多种因素调节胚胎基因组激活。几种类型的全能样细胞已被描述为具有不同的分子特征。在哺乳动物中，细胞在受精时获得全能性。胚胎基因组激活（EGA）发生在小鼠的2-细胞阶段和人类的4-至8-细胞阶段，发生在胚胎细胞全能的时间窗期间，因此认为EGA与全能性的基础机械地相关。导致全能性和EGA建立的分子机制一直是难以捉摸的，然而，最近的进展已经取得了更大的发展潜力的新细胞系的建立和新的低输入高通量技术在胚胎中的应用。这些发现揭示了与其表观遗传组成有关的全能性的几个原则，但也与全能细胞的特征有关。在这篇综述中，我们总结并讨论了目前的观点，探索一些全能性的关键驱动因素，从体外细胞培养模型和体内胚胎发生。
HighlightsCellular models can recapitulate some aspects of embryonic genome activation.Multiple factors regulate embryonic genome activation.Several types of totipotent-like cells have been described with different molecular features.In mammals, cells acquire totipotency at fertilization. Embryonic genome activation (EGA), which occurs at the 2-cell stage in the mouse and 4-to 8-cell stage in humans, occurs during the time window at which embryonic cells are totipotent and thus it is thought that EGA is mechanistically linked to the foundations of totipotency. The molecular mechanisms that lead to the establishment of totipotency and EGA had been elusive for a long time, however, recent advances have been achieved with the establishment of new cell lines with greater developmental potential and the application of novel low-input high-throughput techniques in embryos. These have unveiled several principles of totipotency related to its epigenetic makeup but also to characteristic features of totipotent cells. In this review, we summarize and discuss current views exploring some of the key drivers of totipotency from both in vitro cell culture models and embryogenesis in vivo.