What does the Fos say? Using Fos-based approaches to understand the contribution of stress to substance use disorders.
What does the Fos say? Using Fos-based approaches to understand the contribution of stress to substance use disorders.
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DOI：
10.1016/j.ynstr.2018.05.004
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发表时间：
2018-11
影响因子：
5
通讯作者：
Mantsch JR
中科院分区：
文献类型：
--
作者：
McReynolds JR;Christianson JP;Blacktop JM;Mantsch JR
尽管进行了广泛的研究，但药物成瘾仍然是一个基本上未得到满足的医疗需求。也许治疗成瘾的最大挑战是高再犯率。虽然许多因素可促使戒毒者复吸，但压力的作用尤其成问题，因为压力是无法控制的，在与毒瘾作斗争的人的生活中无处不在。因此，了解压力对药物寻求影响的神经回路对于指导治疗至关重要。旨在定义这种神经回路的临床前研究在一定程度上依赖于使用实验方法，这些方法允许可视化与啮齿动物复发模型中应激诱导的药物寻求相对应的细胞和回路活动。我们所了解的关于介导压力诱发复发的机制的大部分内容都是通过使用立即早期基因cfos或其蛋白产物Fos的表达作为死后活动标记的研究而了解的。在这篇综述中，我们提供了一个概述的啮齿动物模型用于研究应激诱导的复发，并简要总结了什么是已知的基础神经回路之前，描述使用cfos/Fos为基础的方法。除了审查使用这种方法获得的结果，其优点和局限性被认为是。此外，新的技术，利用cfos的表达谱标记和操纵细胞的基础上，他们的活动模式进行了讨论。该综述的目的是指导对旧研究的解释和新研究的设计，这些研究利用cfos/Fos为基础的策略来研究有助于压力相关药物使用的神经回路。
Despite extensive research efforts, drug addiction persists as a largely unmet medical need. Perhaps the biggest challenge for treating addiction is the high rate of recidivism. While many factors can promote relapse in abstinent drug users, the contribution of stress is particularly problematic, as stress is uncontrollable and pervasive in the lives of those struggling with addiction. Thus, understanding the neurocircuitry that underlies the influence of stress on drug seeking is critical for guiding treatment. Preclinical research aimed at defining this neurocircuitry has, in part, relied upon the use of experimental approaches that allow visualization of cellular and circuit activity that corresponds to stressor-induced drug seeking in rodent relapse models. Much of what we have learned about the mechanisms that mediate stressor-induced relapse has been informed by studies that have used the expression of the immediate early gene, cfos, or its protein product, Fos, as post-mortem activity markers. In this review we provide an overview of the rodent models used to study stressor-induced relapse and briefly summarize what is known about the underlying neurocircuitry before describing the use of cfos/Fos-based approaches. In addition to reviewing findings obtained using this approach, its advantages and limitations are considered. Moreover, new techniques that leverage the expression profile of cfos to tag and manipulate cells based on their activity patterns are discussed. The intent of the review is to guide the interpretation of old and design of new studies that utilize cfos/Fos-based strategies to study the neurocircuitry that contributes to stress-related drug use.
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DOI：
10.1523/jneurosci.1393-11.2011
发表时间：
2011-08-03
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Blacktop JM;Seubert C;Baker DA;Ferda N;Lee G;Graf EN;Mantsch JR
通讯作者：
Mantsch JR
社会大脑：社会知识的神经基础。
影响因子：
24.8
作者：
Adolphs, Ralph
通讯作者：
Adolphs, Ralph
DOI：
10.1523/jneurosci.2827-10.2010
发表时间：
2010-12-01
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Briand LA;Vassoler FM;Pierce RC;Valentino RJ;Blendy JA
通讯作者：
Blendy JA
影响因子：
25
作者：
Bossert, Jennifer M.;Stern, Anna L.;Theberge, Florence R. M.;Cifani, Carlo;Koya, Eisuke;Hope, Bruce T.;Shaham, Yavin
通讯作者：
Shaham, Yavin
影响因子：
3.4
作者：
Capriles, N;Rodaros, D;Stewart, J
通讯作者：
Stewart, J