Fine structure of cultured epithelial cells derived from voided urine of normal adults
Fine structure of cultured epithelial cells derived from voided urine of normal adults
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正常成人尿液培养上皮细胞的精细结构
DOI：
10.1007/bf02892835
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
L. Koss
中科院分区：
文献类型：
--
作者：
Siamak Shokri;F. Herz;L. Koss
上皮细胞的培养可以从正常成人的尿液沉淀物开始。不同形态的细胞形成紧密或松散的集落。超微结构显示增殖细胞形成丰富的桥粒，紧密连接和致密层形成不完善，所有这些都是典型的上皮细胞。一些细胞衬有特征性的不对称单位膜，从而证实了培养物的尿路上皮来源。在某些细胞的表面附近观察到了奇特的、似乎是细胞质起源的多泡体，这显然是迄今为止没有描述过的。尿细胞培养系统是用于诊断和研究目的的潜在有用工具。
SummaryCultures of epithelial cells can be initiated with the sediment of voided urine of normal adults. Tightly or loosely packed colonies were formed by cells of diverse morphologic configuration. Ultrastructural studies revealed that the proliferating cells formed abundant desmosomes, imperfectly formed tight junctions and lamina densa, all typical of epithelial cells. Some cells were lined by the characteristic asymmetric unit membrane, thus confirming the urothelial derivation of the cultures. Peculiar, apparently hitherto not described multivesicular bodies, seemingly of cytoplasmic origin, were observed near the surfaces of some cells. The urinary cell culture system is a potentially useful tool for diagnostic and research purposes.
大鼠膀胱正常上皮细胞、内皮细胞和成纤维细胞的体外分离和表征。
影响因子：
2.6
作者：
Pauli,BU;Anderson,SN;Memoli,VA;Kuettner,KE
通讯作者：
Kuettner,KE