Influence of p53 and genetic background on prenatal oogenesis and oocyte attrition in mice
Influence of p53 and genetic background on prenatal oogenesis and oocyte attrition in mice
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p53 和遗传背景对小鼠产前卵子生成和卵母细胞损耗的影响
DOI：
10.1093/humrep/dep022
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发表时间：
2009-06-01
影响因子：
6.1
通讯作者：
Hartshorne, G. M.
中科院分区：
文献类型：
--
作者：
Ghafari, F.;Pelengaris, S.;Hartshorne, G. M.
减数分裂进程和存活到出生的卵母细胞数量决定了卵巢储备，但产前卵子发生的控制知之甚少。我们研究了遗传背景和p53基因对小鼠卵子发生的影响，分析了B6 CBf 1和B6 CBf 2小鼠在交配后15.5 - 21天（dpc）和p53（一种肿瘤抑制基因）敲除、杂合子和野生型小鼠在15.5 - 16 dpc的胎儿和新生儿卵巢。通过标记联会复合体蛋白3来鉴定减数分裂前期Ⅰ（MPI）的卵母细胞，并根据轴向元件的出现来划分MPI的具体阶段。用酶切聚腺苷二磷酸核糖聚合酶（PARP-1）和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）分别检测细胞凋亡和DNA断裂。
Meiotic progression, and the number of oocytes surviving to birth, determine the ovarian reserve, yet the control of prenatal oogenesis is poorly understood. We investigated the effects of genetic background and p53 upon oogenesis in mice.Fetal and neonatal ovaries were analysed in B6CBf1 and B6CBf2 mice from 15.5 to 21 days post-coitum (dpc) and p53 (a tumour suppressor gene) knockout, heterozygous and wild-type mice from 15.5 to 16 dpc. Oocytes in meiotic prophase I (MPI) were identified by labelling synaptonemal complex protein 3, and the specific stage of MPI was classified by the appearance of axial elements. Apoptosis and DNA breaks were assessed by cleaved poly-(ADP-ribose) polymerase (PARP-1) and terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling (TUNEL), respectively.The leptotene, zygotene and pachytene stages were earlier in f1 than f2 generations with significant differences at all stages (P