Computational segmentation of collagen fibers in bone matrix indicates bone quality in ovariectomized rat spine
Computational segmentation of collagen fibers in bone matrix indicates bone quality in ovariectomized rat spine
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DOI：
10.1007/s00774-017-0844-5
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发表时间：
2018-05-01
影响因子：
3.3
通讯作者：
El Khassawna, Thaqif
中科院分区：
文献类型：
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作者：
Daghma, Diaa Eldin S.;Malhan, Deeksha;El Khassawna, Thaqif
骨丢失根据疾病和年龄而变化，这些变化影响骨细胞和细胞外基质。骨质疏松大鼠模型被广泛研究，以评估骨的力学和结构特性，然而，骨基质蛋白及其不一致的调节病变和老化骨往往被忽视。本研究考虑了16月龄时卵巢切除大鼠（OVX）与对照大鼠（Sham）的脊柱基质特性。骨组织致密性较差，1型胶原（Col1）分布不均匀，骨细胞形态发生变化。有趣的是，在OVX脊柱的血管附近发现了脱矿斑。有机基质结构进行了研究，使用计算分割的胶原纤维的性质。与老年骨相比，病变骨显示出更长的原纤维和更小的取向角。该研究显示了量化透射电子显微镜图像来预测骨组织的机械性能的潜力。
Bone loss varies according to disease and age and these variations affect bone cells and extracellular matrix. Osteoporosis rat models are widely investigated to assess mechanical and structural properties of bone; however, bone matrix proteins and their discrepant regulation of diseased and aged bone are often overlooked. The current study considered the spine matrix properties of ovariectomized rats (OVX) against control rats (Sham) at 16 months of age. Diseased bone showed less compact structure with inhomogeneous distribution of type 1 collagen (Col1) and changes in osteocyte morphology. Intriguingly, demineralization patches were noticed in the vicinity of blood vessels in the OVX spine. The organic matrix structure was investigated using computational segmentation of collagen fibril properties. In contrast to the aged bone, diseased bone showed longer fibrils and smaller orientation angles. The study shows the potential of quantifying transmission electron microscopy images to predict the mechanical properties of bone tissue.