Lab-on-a-Drone: Toward Pinpoint Deployment of Smartphone-Enabled Nucleic Acid-Based Diagnostics for Mobile Health Care.
Lab-on-a-Drone: Toward Pinpoint Deployment of Smartphone-Enabled Nucleic Acid-Based Diagnostics for Mobile Health Care.
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DOI：
10.1021/acs.analchem.5b04153
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发表时间：
2016-05-03
影响因子：
7.4
通讯作者：
Ugaz VM
中科院分区：
文献类型：
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作者：
Priye A;Wong S;Bi Y;Carpio M;Chang J;Coen M;Cope D;Harris J;Johnson J;Keller A;Lim R;Lu S;Millard A;Pangelinan A;Patel N;Smith L;Chan K;Ugaz VM
我们介绍了一种便携式生化分析平台，用于使用消费级四轴飞行器无人机快速现场部署基于核酸的诊断。这种方法利用了用单个加热器等温进行聚合酶链式反应（PCR）的能力，使得系统能够使用标准5 V USB电源来操作，该USB电源为移动的设备供电（通过电池、太阳能或手动曲柄动作）。使用智能手机摄像头和集成的图像分析应用程序实现时间分辨荧光检测和定量。通过3D打印的卡扣式附件将无人机的电机用作离心机，实现标准样品制备。这些进步使得建立一个完整的DNA/RNA分析系统成为可能，成本为50美元。我们的仪器坚固耐用，功能多样，能够精确地将复杂的诊断部署到分布式现场。通过智能手机连接进行快速飞行检测的能力消除了样本收集和分析之间的延迟，因此可以在几分钟内提供测试结果，这表明基于无人机的系统在货物运输和成像之外发挥更广泛和更复杂作用的新可能性。
We introduce a portable biochemical analysis platform for rapid field deployment of nucleic acid-based diagnostics using consumer-class quadcopter drones. This approach exploits the ability to isothermally perform the polymerase chain reaction (PCR) with a single heater, enabling the system to be operated using standard 5 V USB sources that power mobile devices (via battery, solar, or hand crank action). Time-resolved fluorescence detection and quantification is achieved using a smartphone camera and integrated image analysis app. Standard sample preparation is enabled by leveraging the drone’s motors as centrifuges via 3D printed snap-on attachments. These advancements make it possible to build a complete DNA/RNA analysis system at a cost of ∼$50 ($US). Our instrument is rugged and versatile, enabling pinpoint deployment of sophisticated diagnostics to distributed field sites. This capability is demonstrated by successful in-flight replication of Staphylococcus aureus and λ-phage DNA targets in under 20 min. The ability to perform rapid in-flight assays with smartphone connectivity eliminates delays between sample collection and analysis so that test results can be delivered in minutes, suggesting new possibilities for drone-based systems to function in broader and more sophisticated roles beyond cargo transport and imaging.