Expression of keratinocyte growth factor and its receptor in human endometrial cancer in cooperation with steroid hormones.
Expression of keratinocyte growth factor and its receptor in human endometrial cancer in cooperation with steroid hormones.
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DOI：
10.3892/ijo.32.3.565
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发表时间：
2008-03
影响因子：
5.2
通讯作者：
A. Ishikawa;M. Kudo;N. Nakazawa;M. Onda;T. Ishiwata;T. Takeshita;Z. Naito
中科院分区：
文献类型：
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作者：
A. Ishikawa;M. Kudo;N. Nakazawa;M. Onda;T. Ishiwata;T. Takeshita;Z. Naito
角化细胞因子（KGF）及其受体（KGFR）参与组织发育和修复。我们研究了KGF和KGFR在人子宫内膜组织和细胞中的表达和功能，以及KGFR与雌激素和孕激素的关系。在处于分泌期的非癌性人子宫内膜组织中，检测到KGF在腺体、基质细胞和螺旋动脉平滑肌细胞中具有较强的免疫反应性；然而，在增殖期组织中，KGF或KGFR的免疫反应性较弱或不存在。32例子宫内膜样腺癌中，KGF和KGFR染色均为阳性（分别为90.6%和71.9%）。然后，我们利用表达雌激素受体（ER）和孕激素受体（PR）的石川高分化人子宫内膜癌细胞系，研究了KGF和KGFR在雌激素和孕激素联合作用下的表达，发现雌激素上调石川细胞的KGFR表达，并且这种上调在黄体酮的共同作用下明显增强。我们还观察到，KGF给药后，KGFR表达上调，刺激ERK1/2磷酸化和细胞对纤维连接蛋白的粘附。本文讨论了激素刺激的KGF-KGFR表达在调节与人子宫内膜癌相关的细胞行为中的意义。
The keratinocyte factor (KGF) and its receptor (KGFR) are implicated in tissue development and repair. We studied the expression and functions of KGF and KGFR in association with estrogen and progesterone in human endometrial tissues and cells. In non-cancerous human endometrial tissues in the secretory phase, a strong immunoreactivity of KGF in glands, stromal cells, and smooth muscle cells of spiral arteries was detected; however, in proliferative-phase tissues, the immunoreactivity of KGF or KGFR was weak or absent. Most of the 32 endometrioid adenocarcinoma cases showed positive KGF and KGFR stainings (90.6 and 71.9%, respectively). We then studied, using Ishikawa well-differentiated human endometrial cancer cell line that expresses estrogen receptor (ER) and progesterone receptor (PR), the expression of KGF and KGFR in conjunction with estrogen and progesterone, and observed that the KGFR expression of Ishikawa cells was upregulated by estrogen and that this upregulation was markedly enhanced by the coadministration of progesterone. We also observed that KGF administration to cells, with KGFR upregulated expression, stimulated ERK1/2 phosphorylation and cell adhesion to fibronectin. The implications of the hormone-stimulated KGF-KGFR expressions in the regulation of cell behavior associated with human endometrial cancer are discussed.