Effects of room temperature and cold storage on the metabolic and haemostatic properties of whole blood for acute normovolaemic haemodilution.
Effects of room temperature and cold storage on the metabolic and haemostatic properties of whole blood for acute normovolaemic haemodilution.
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DOI：
10.1371/journal.pone.0267980
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
急性等容性血液稀释（ANH）作为一种血液保护技术，避免了异体输血的需要。O型全血（WB）冷藏在军事领域的历史实践推广了冷藏WB输注治疗急性出血。在这项研究中，我们比较了室温（RT）和冷藏长达24小时的WB对ANH的凝血性能的影响。将从12名男性志愿者中采集的每份WB样品分为两部分，一部分储存在室温下，另一部分冷藏24小时。测量全血细胞计数（CBC）、血气水平和凝血特征，并在初始采集时间点（基线）和初始采集后6、12和24小时进行旋转血栓弹性测定（ROTEM）测量。冷保存的全血对花生四烯酸和二磷酸腺苷诱导的血小板聚集反应的保护作用优于室温保存的全血。凝血酶受体激活肽6诱导的血小板聚集反应在储存24小时后与基线相比，在所有样品中均显著降低。与冷藏样品相比，在室温下储存的WB中的乳酸水平在储存6小时后显著增加。在CBC、凝血参数和ROTEM变量方面，冷藏和RT储存的WB样本之间无显著差异。WB的ANH存储在冰箱中显示出更好的代谢特性后，6小时的存储和更好的聚集反应后，12小时的存储比WB存储在RT。
Acute normovolaemic haemodilution (ANH), as a blood-conservation technique, avoids the need for allogeneic blood transfusions. The historic practice of cold-storing type-O whole blood (WB) in military fields popularised the transfusion of refrigerated WB to treat acute bleeding. In this study, we compared the effects of room temperature (RT) and refrigeration up to 24 hours on the coagulation properties of WB for ANH. Each WB sample, collected from 12 male volunteers, was divided into two parts, one stored at RT and the other refrigerated for 24 hours. Complete blood counts (CBC), blood gas levels, and coagulation profiles were measured, and rotational thromboelastometry (ROTEM) measurements were performed at the initial collection time point (baseline) and at 6, 12, and 24 hours after initial collection. The preservation of platelet aggregation response induced by arachidonic acid and adenosine diphosphate was better in cold-stored WB compared to that in RT-stored WB. The platelet aggregation response induced by thrombin receptor-activating peptide 6 was significantly decreased in all samples after 24 hours of storage when compared with that at baseline. The lactate levels in WB stored at RT increased significantly after 6 hours of storage compared to that of cold-stored samples. There were no significant differences in CBC, coagulation parameters, and ROTEM variables between the cold-stored and RT-stored WB samples. WB for ANH stored in the refrigerator showed better metabolic characteristics after 6 hours of storage and better aggregation response after 12 hours of storage than WB stored at RT.
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影响因子：
3.3
作者：
Ranucci, Marco;Di Dedda, Umberto;Baryshnikova, Ekaterina
通讯作者：
Baryshnikova, Ekaterina
DOI：
10.1097/shk.0000000000000082
发表时间：
2014-05
期刊：
Shock (Augusta, Ga.)
影响因子：
--
作者：
Reddoch KM;Pidcoke HF;Montgomery RK;Fedyk CG;Aden JK;Ramasubramanian AK;Cap AP
通讯作者：
Cap AP
影响因子：
5.7
作者：
Lu SY;Konig G;Yazer MH;Brooks JP;Chen YF;Jeong JH;Waters JH
通讯作者：
Waters JH
影响因子：
20.3
作者：
MANNO, CS;HEDBERG, KW;NORWOOD, WI
通讯作者：
NORWOOD, WI
影响因子：
2.9
作者：
Lozano, ML;Rivera, J;Vicente, V
通讯作者：
Vicente, V