A primary study on genes with selected mutations by in vitro passage of Chlamydia muridarum strains
A primary study on genes with selected mutations by in vitro passage of Chlamydia muridarum strains
复制标题
鼠衣原体菌株体外传代选择性突变基因的初步研究
DOI：
10.1093/femspd/ftz017
复制
发表时间：
2019
影响因子：
3.3
通讯作者：
Zhong Guangming
中科院分区：
文献类型：
--
作者：
Zhou Zhou;Liu Na;Wang Yingzi;Emmanuel Arthur Wirekoh;You Xiaoxing;Liu Jiulin;Li Zhongyu;Wu Yimou;Zhong Guangming
目的：研究小鼠衣原体（Chlamydia muridarum，C.方法采用全基因组测序和空斑形成试验，对C.建立了鼠源亲本和传代菌株，并分离出表达某些基因型的等基因克隆。获得了具有单基因差异的菌株。根据预测结果，筛选出tc 0412的单基因差异有价值的菌株，对tc 0668和tc 0237进行了体外和体内生物学特性和毒力分析（T28 T/B3型）与非突变型tc 0668基因和点突变为G797659 T（Q117 E）的tc 0237基因相匹配，可能由于缺乏质粒而使衣原体生长缓慢。突变株tc 0668可引起小鼠输卵管积水的高发病率，而突变株tc 0668具有G797659 T点突变则显著减弱。与非突变型tc 0237相比，突变型tc 0237菌株感染时离心依赖性降低。结论这些基因的鉴定和鉴定有助于全面了解衣原体的致病机制。
ObjectiveThis study is to investigate the functions of newly discovered genes inChlamydia muridarum(C. muridarum) strains with single gene differences.MethodsUsing whole genome sequencing and plaque formation assays,C. muridarumparental and passaging strains were established, and the isogenic clones expressing certain genotypes were isolated. Strains with single gene differences were obtained. Based on prediction, the valuable strains with single gene differences of tc0412, tc0668 or tc0237 were subjected to thein vitroandin vivoexperiments for biological characterization and virulence analysis.ResultsInsertional-472840Tmutation of the tc0412 gene (T28T/B3 type) matching with the nonmutant tc0668 gene and tc0237 gene with point mutations G797659T (Q117E) might slow the growth ofChlamydiadue to the lack of a plasmid. The nonmutant tc0668 in the strain might induce a high incidence of hydrosalpinx in mice, while tc0668 with a G797659T point mutation was significantly attenuated. Compared with the nonmutant tc0237, the strains containing mutant tc0237 were characterized by reduced centrifugation dependence during infection.ConclusionThe identification and characterization of these genes might contribute to the comprehensive understanding of the pathogenic mechanism ofChlamydia.