Nerve fibres are required to evoke a contact sensitivity response in mice
Nerve fibres are required to evoke a contact sensitivity response in mice
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DOI：
10.1111/j.1365-2567.2004.01786.x
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发表时间：
2004-01-01
期刊：
影响因子：
6.4
通讯作者：
Miyan, JA
中科院分区：
文献类型：
--
作者：
Beresford, L;Orange, O;Miyan, JA
以往的研究表明，皮肤的真皮和表皮含有丰富的与朗格汉斯细胞密切相关的神经纤维。我们已经研究了这些神经末梢是否是诱导和诱发接触敏感性(CS)反应所必需的。局部应用普通或多肽(降钙素基因相关肽和P物质)特异性神经毒素来破坏皮肤部位的神经纤维，然后用来诱导或诱发CS反应。神经纤维的消除消除了特定CS反应的诱导阶段和效应阶段。去神经治疗不会破坏局部朗格汉斯细胞，也不会阻止它们向淋巴结迁移。局部CS反应也被辣椒素敏感神经纤维的系统性删除所取消，这表明反应的丧失不是非特异性的，而是与特定神经纤维的丢失有关。结果表明，肽能神经纤维是诱导CS反应所必需的，可能对免疫系统的正常功能至关重要。
Previous work has indicated that the dermis and epidermis of skin contains abundant nerve fibres closely associated with Langerhans' cells. We have investigated whether these nerve endings are necessary for inducing and evoking a contact sensitivity (CS) response. Topical application of a general or a peptide (calcitonin gene-related peptide and substance P)-specific neurotoxin was employed to destroy the nerve fibres at skin sites subsequently used to induce or evoke the CS response. Elimination of nerve fibres abolished both induction and effector stages of the specific CS response. Denervation did not destroy the local Langerhans' cells, which were observed in increased numbers, or prevent them from migrating to lymph nodes. The local CS response was also abolished by systemic deletion of capsaicin-sensitive nerve fibres, suggesting that the loss of response was not non-specific but associated with the loss of specific nerve fibres. The results indicate that peptidergic nerve fibres are required to elicit a CS response and may be vital to the normal function of the immune system.