The minimum significant ratio: A statistical parameter to characterize the reproducibility of potency estimates from concentration-response assays and estimation by replicate-experiment studies
The minimum significant ratio: A statistical parameter to characterize the reproducibility of potency estimates from concentration-response assays and estimation by replicate-experiment studies
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DOI：
10.1177/1087057105285611
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发表时间：
2006-04-01
影响因子：
--
通讯作者：
Smith, GF
中科院分区：
文献类型：
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作者：
Eastwood, BJ;Farmen, MW;Smith, GF
作者通过实例表明，用于验证单浓度高通量筛选可靠性的程序，如信号窗口和Z‘因子，不能确保从浓度响应分析中估计效价的足够可靠性。他们开发了最小显着比作为统计参数来表征在同一实验中运行的两种化合物之间的折叠变化，这可以被认为是真正的差异，并使用这个参数来表征分析的可靠性。他们采用了Bland和Altman所描述的方法，开发了一套简单的两个实验来估计最小显着比，并表明该方案可以识别缺乏重复性的分析。然后将这些方法扩展到验证由多个实验室运行的同一检测的等效性。
The authors show by illustration that procedures used to validate the reliability of single-concentration high-throughput screens such as the signal window and Z' factor do not ensure sufficient reliability in potency estimates from concentration response assays. They develop the minimum significant ratio as a statistical parameter to characterize the fold change between 2 compounds run in the same experiment that can be considered a real difference and use this parameter to characterize the reliability of the assay. They adapt methods described by Bland and Altman to develop a simple set of 2 experiments to estimate the minimum significant ratio and show that this protocol can identify assays that lack reproducibility. The methods are then extended to validate the equivalency of the same assay run by multiple laboratories.