Direct comparison of radiolabeled probes FMAU, FHBG, and FHPG as PET imaging agents for HSV1-tk expression in a human breast cancer model.
Direct comparison of radiolabeled probes FMAU, FHBG, and FHPG as PET imaging agents for HSV1-tk expression in a human breast cancer model.
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放射性标记探针 FMAU、FHBG 和 FHPG 作为 PET 显像剂直接比较人类乳腺癌模型中 HSV1-tk 的表达。
DOI：
10.1162/15353500200403160
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发表时间：
2004
影响因子：
2.8
通讯作者：
Conti,PeterS
中科院分区：
文献类型：
--
作者：
Alauddin,MianM;Shahinian,Atranik;Gordon,ErlindaM;Conti,PeterS
2′-Deoxy-2′-fluoro-5-methyl-1-β-d-arabinofuranosyluracil (FMAU), 9-(4-fluoro-3-hydroxy-methyl-butyl)guanine (FHBG) and 9-[(3-fluoro-1-hydroxy-2-propoxy)methyl]-guanine (FHPG) have been evaluated in a human breast cancer model as potential radiotracers for PET imaging of HSV1-tk gene expression. In vitro accumulation of [14C]FMAU, [18F]FHBG, and [18F]FHPG in HSV1-tk-expressing cells was 14- to 16-fold (p< .001), 9- to 13-fold (p< .001), and 2- to 3-fold (p< .05) higher than tk-negative control cells, respectively, between 30 and 240 min. Accumulation of FMAU and FHBG in vector-transduced cells was 10- to 14-fold and 6- to 10-fold higher than wild-type cells, respectively. At 2 hr, uptake of [14C]FMAU in tk-positive cells was 6.3-fold and 60-fold higher than [18F]FHBG and [18F]FHPG, respectively. In vivo, tumor uptake of [14C]FMAU in HSV1-tk-expressing cells was 3.7-fold and 5.5-fold (p< .001) higher than tk-negative control cells at 1 and 2 hr, respectively. Tumor uptake of [18F]FHBG was 4.2-fold and 12.6-fold higher (p< .001) than tk-negative cells at the same time points. Incorporation of [14C]FMAU in tk-positive tumor was 18-fold and 24-fold higher (p< .001) than [18F]FHBG at 1 and 2 hr, respectively. Micro-PET images support the biodistribution results and indicate that both [18F]FMAU and [18F]FHBG are useful for imaging HSV1-tk expression in breast cancer. Although FMAU demonstrates higher total incorporation (%dose/g) in tumor tissue compared with the other tracers, FHBG is superior in terms of specific accumulation in transfected cells at later time points.
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DOI：
--
发表时间：
2002-08
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
J. Tjuvajev;M. Doubrovin;T. Akhurst;S. Cai;J. Balatoni;M. Alauddin;R. Finn;W. Bornmann;H. Thaler;P. Conti;R. Blasberg
通讯作者：
J. Tjuvajev;M. Doubrovin;T. Akhurst;S. Cai;J. Balatoni;M. Alauddin;R. Finn;W. Bornmann;H. Thaler;P. Conti;R. Blasberg
DOI：
--
发表时间：
2001
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
M. Iyer;J. R. Barrio;M. Namavari;Eileen Bauer;N. Satyamurthy;Khoi Nguyen;T. Toyokuni;Michael E. Phelps;Harvey R. Herschman;S. Gambhir
通讯作者：
M. Iyer;J. R. Barrio;M. Namavari;Eileen Bauer;N. Satyamurthy;Khoi Nguyen;T. Toyokuni;Michael E. Phelps;Harvey R. Herschman;S. Gambhir
基于正电子发射断层扫描的转基因表达成像，由体内复制条件性溶瘤单纯疱疹病毒 1 型突变载体介导。
DOI：
--
发表时间：
2001
期刊：
Cancer research.
影响因子：
--
作者：
Jacobs,A;Tjuvajev,JG;Dubrovin,M;Akhurst,T;Balatoni,J;Beattie,B;Joshi,R;Finn,R;Larson,SM;Herrlinger,U;Pechan,PA;Chiocca,EA;Breakefield,XO;Blasberg,RG
通讯作者：
Blasberg,RG
影响因子：
4.8
作者：
Fu, DX;Kobayashi, M;Lin, L
通讯作者：
Lin, L
影响因子：
1.8
作者：
Alauddin, MM;Conti, PS;Fissekis, JD
通讯作者：
Fissekis, JD