ATP synthase subunit e is a shrimp growth-associated breeding marker
ATP synthase subunit e is a shrimp growth-associated breeding marker
复制标题
DOI：
10.1016/j.ygeno.2022.110410
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Fan Wang
中科院分区：
文献类型：
--
作者：
Yucheng Lu;Qingyun Liu;Zhiqi Huang;Xiuli Chen;Chunling Yang;Yueling Zhang;Yongzhen Zhao;Fan Wang
Penaeus vannamei is one of the most popular aquaculture species in the world. This species is featured with its fast-growing and delicious taste, which drives people develop various strains. During this process identification of trait-associated markers could effectively increase breeding efficiency. Driven by this, we tried to screen fast-growing key regulators via a FACS-based high throughput method, in which 2-NBDG was applied as a fluorescent indicator for direct glucose uptake measurement. Totally six candidate genes were screened out followed by in vitro validation in 293T cells. After that, the correlation between these genes and shrimp growing was further verified in a hybrid lineage. The expression level of two genes including ATP synthase subunit e and inhibitor of apoptosis protein showed some correlation with shrimp growth speed. Furthermore, we tested these two candidate markers in various lineages and confirmed that ATP synthase subunit e could be a shrimp growth-associated breeding marker.
