Identification and characterization of microRNAs in the zoonotic fluke Fasciolopsis buski
Identification and characterization of microRNAs in the zoonotic fluke Fasciolopsis buski
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DOI：
10.1007/s00436-016-4995-4
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发表时间：
2016-06-01
影响因子：
2
通讯作者：
Chen, Jia-Xu
中科院分区：
文献类型：
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作者：
Chen, Mu-Xin;Hu, Wei;Chen, Jia-Xu
猪片吸虫是一种食源性人畜共患寄生虫，由水生植物传播，猪和人为最终宿主。本研究的目的是通过Solexa深度测序和生物信息学分析来鉴定这种寄生虫的microRNA(MiRNA)图谱。从成虫身上获得了大约1200万份高质量的读物。在miRBase数据库中共发现了286个miRNA候选序列，其中24个miRNA候选序列保守。通过茎环逆转录聚合酶链式反应(RT-PCR)鉴定了3个新的miRNAs。本研究中发现的miRNAs属于13个成员表现出高度偏见的家族。在保守的miRNAs的起始和中间，尤其是在第2、6和13位，鸟嘌呤(G)是占主导地位的核苷酸。据我们所知，这是首次报道白粉菌的miRNA图谱，为进一步研究白粉菌miRNAs的功能奠定了基础。
Fasciolopsis buski is a food-borne zoonotic parasite which is transmitted by aquatic plants, with pigs and humans as the definitive hosts. The objective of the present study was to characterize the microRNA (miRNA) profiles of this parasite by Solexa deep sequencing and bioinformatic analysis. Approximately 12 million high-quality reads were obtained from adult F. buski. A total of 286 miRNA candidates were found and 24 miRNA candidates were conserved miRNAs in the miRBase database. Three novel miRNAs were identified and confirmed by stem-loop reverse transcriptase polymerase chain reaction (RT-PCR). The miRNAs found in the present study belong to 13 families whose members showed high bias. The guanine (G) was the dominant nucleotide at the beginning and middle of the conserved miRNAs, particularly at the positions of 2nd, 6th, and 13th. To our knowledge, this is the first report of the miRNA profiles of F. buski, which would lay a foundation for further functional studies of miRNAs of F. buski.