Electronic Supplementary Information Chloride Accelerated Fenton Chemistry for Ultrasensitive and Selective Colorimetric Detection of Copper
Electronic Supplementary Information Chloride Accelerated Fenton Chemistry for Ultrasensitive and Selective Colorimetric Detection of Copper
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通讯作者：
Zhi Shan;Mingsheng Lu;Li Wang;Bruce MacDonald;Judy MacInnis;M. Mkandawire;K. Oakes;Xu Zhang
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作者：
Zhi Shan;Mingsheng Lu;Li Wang;Bruce MacDonald;Judy MacInnis;M. Mkandawire;K. Oakes;Xu Zhang
（3-乙基苯并噻唑啉-6-磺酸）（ABTS）、二氨基联苯胺（DAB）、2-（N-吗啉代）乙磺酸（MES）获自Sigma-Aldrich。愈创木酚、邻苯二胺（OPD）、邻联茴香胺（ODA）和对苯二甲酸（TPA，≥98%）来自Alfa Aesar。在DMSO（50 mM）中制备，并在使用前在-70 °C下避光储存。为了确保没有其他金属离子的痕量污染，在使用前，将NaCl（5 M）和HCl（10 mM）溶液在室温下用Chelex 100树脂（终浓度1%w/v）处理过夜。和甘露醇是分析试剂级的并且从Sigma-Aldrich获得;丙醇（99.9%）从Fisher Scientific购买，并且叔丁醇（TBA，99.5%）从Alfa Aesar获得;所有这些都不经进一步纯化而使用。使用Barnstead NANO-pure系统（Thermo Scientific）制备的纳米纯水（18 MΩ）用于所有实验。值得强调的是，使用正确的缓冲液对于这项研究至关重要，因为已知铜与许多不同的阴离子相互作用。由于MES缓冲液在芬顿反应中与铜1的相互作用低，因此在我们的工作中使用MES缓冲液。此外，由于自由基的消耗较低，因此使用较低浓度的MES，从而有利于TMB-H 2 O 2反应，并在铜检测中实现高灵敏度（图S21）。仪器：通过M1000 Pro酶标仪（TECAN，USA）使用圆底透明96孔聚苯乙烯或平底黑色96孔聚苯乙烯板（COSTAR，USA）进行比色和荧光测量。还使用DR 5000 UV-Vis分光光度计（哈赫，德国）获得吸光度（1 cm比色皿），用于计算初始反应速率。对于裸眼Cu（II）检测，用数码相机记录溶液颜色。使用Mineralight UVGL-25灯（San Gabriel，USA）在366 nm下进行UV照射。用与5973惰性质谱仪偶联的Agilent 6890 N GC进行氯化化合物的鉴别。用于ChemComm的真实的电子补充材料（ESI）中的铜浓度。
(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), diaminobenziidine (DAB), 2-(N-morpholino)ethanesulfonic acid (MES), were obtained from Sigma-Aldrich. Guaiacol, o-phenylenediamine (OPD), o-dianisidine (ODA), and terephthalic acid (TPA, ≥98%) were received from Alfa Aesar. was prepared in DMSO (50 mM) and stored in the dark at-70°C prior to use. To ensure no trace contamination of other metal ions, NaCl (5 M) and HCl (10 mM) solutions were treated with Chelex100 resin (final concentration 1% w/v) overnight under room temperature before use. , and mannitol were of analytical reagent grade and obtained from Sigma-Aldrich; propanol (99.9%) was purchased from Fisher Scientific, and tert-butyl alcohol (TBA, 99.5%) was obtained from Alfa Aesar; all were used without further purification. Nanopure water (18 MΩ) prepared with a Barnstead NANO-pure system (Thermo Scientific) was used for all experiments. It is worth emphasizing that the use of the right buffer is critical for this research, since copper is known to interact with many different anions. MES buffer was used in our work due to its low interaction with copper 1 in Fenton reactions. Besides, lower concentration of MES was used due to lower depletion of free radicals, thus favoring for TMB-H 2 O 2 reaction and achieving high sensitivity in copper detection (Fig. S21). Instrumentation: Colorimetric and fluorescence measurements were performed by a M1000 Pro plate reader (TECAN, USA) using either round bottom clear 96-well polystyrene or flat bottom black 96-well polystyrene plates (COSTAR, USA). A DR 5000 UV-Vis spectrophotometer (HACH, Germany) was also used to obtain the absorbance (1 cm cuvette) for calculation of initial reaction rates. For naked eye Cu(II) detection, solution color was recorded with a digital camera. UV irradiation was performed using mineralight UVGL-25 lamp (San Gabriel, USA) at 366 nm. Identification of chlorinated compound was performed with an Agilent 6890 N GC coupled to a 5973 Inert Mass Spectrometer. Copper concentration in real Electronic Supplementary Material (ESI) for ChemComm.