Genome-Wide Analysis and Cloning of the Apple Stress-Associated Protein Gene Family Reveals MdSAP15, Which Confers Tolerance to Drought and Osmotic Stresses in Transgenic Arabidopsis
Genome-Wide Analysis and Cloning of the Apple Stress-Associated Protein Gene Family Reveals MdSAP15, Which Confers Tolerance to Drought and Osmotic Stresses in Transgenic Arabidopsis
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苹果胁迫相关蛋白基因家族的全基因组分析和克隆揭示了 MdSAP15，它赋予转基因拟南芥对干旱和渗透胁迫的耐受性
DOI：
10.3390/ijms19092478
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发表时间：
2018-09-01
影响因子：
5.6
通讯作者：
Ma, Fengwang
中科院分区：
文献类型：
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作者：
Dong, Qinglong;Duan, Dingyue;Ma, Fengwang
逆境相关蛋白（Stress-associated proteins，SAP）是一类新的含A20/AN 1锌指结构域的蛋白质，是提高植物抗逆能力的重要靶标。然而，SAP基因家族及其在重要果树苹果中的生物学功能尚不清楚。我们在苹果中对该基因家族进行了全基因组分析和克隆，并确定MdSAP 15的过表达增强了拟南芥植物的耐旱性。我们在苹果基因组中鉴定了30个SAP基因。系统发育分析揭示了该家族中的两个主要群体。通过序列比对和三维结构、遗传学、基因组学结构和保守结构域的分析表明，苹果SAP具有高度的结构保守性。全面的qRT-PCR分析发现不同组织中MdSAP的各种表达模式，并响应于缺水。转基因分析表明，MdSAP 15在转基因拟南芥中的过表达显着增强了它们对渗透胁迫和干旱胁迫的耐受性。我们的研究结果表明，SAP基因在植物物种中高度保守，MdSAP 15可以作为一个目标基因的基因工程方法，以提高耐旱性。
Stress-associated proteins (SAPs) are novel A20/AN1 zinc finger domain-containing proteins that are now favorable targets to improve abiotic stress tolerance in plants. However, the SAP gene family and their biological functions have not been identified in the important fruit crop apple (Malus × domestica Borkh.). We conducted a genome-wide analysis and cloning of this gene family in apple and determined that the overexpression of MdSAP15 enhances drought tolerance in Arabidopsis plants. We identified 30 SAP genes in the apple genome. Phylogenetic analysis revealed two major groups within that family. Results from sequence alignments and analyses of 3D structures, phylogenetics, genomics structure, and conserved domains indicated that apple SAPs are highly and structurally conserved. Comprehensive qRT-PCR analysis found various expression patterns for MdSAPs in different tissues and in response to a water deficit. A transgenic analysis showed that the overexpression of MdSAP15 in transgenic Arabidopsis plants markedly enhanced their tolerance to osmotic and drought stresses. Our results demonstrate that the SAP genes are highly conserved in plant species, and that MdSAP15 can be used as a target gene in genetic engineering approaches to improve drought tolerance.