CYTOPLASMIC SYNTHESIS OF HISTONES IN HELA CELLS AND ITS TEMPORAL RELATIONSHIP TO DNA REPLICATION
CYTOPLASMIC SYNTHESIS OF HISTONES IN HELA CELLS AND ITS TEMPORAL RELATIONSHIP TO DNA REPLICATION
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DOI：
10.1073/pnas.57.2.409
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发表时间：
1967-01-01
影响因子：
11.1
通讯作者：
BORUN, TW
中科院分区：
文献类型：
--
作者：
ROBBINS, E;BORUN, TW
在同步的HeLa细胞中，组蛋白的起始和DNA合成之间存在精确的相关性。在G1和S期对细胞进行脉冲追踪标记，并用色氨酸和赖氨酸双重标记的细胞质多体进行检查，结果表明小多体的细胞质上产生了结石。DNA抑制剂阿拉伯糖胞嘧啶和5氟脱氧胆碱在S期加入时可选择性地破坏这些聚合体，但在G1期加入时对相同大小的小聚体没有影响。
In synchronized HeLa cells there is a precise correlation between the initiation of histone and DNA synthesis. Pulse-chase labeling of cells in G1 and S and examination of cytoplasmic polysomes doubly labeled with tryptophan and lyslne have shown that hlstones are made in cytoplasm on small polysomes. The DNA inhibitors cytosine arabinoslde and 5 fluorodeoxyurldlne cause the selective disruption of these when they are added during S, but have no effect on small polysomes of the same size when added during G1.