SlMYB102, an R2R3-type MYB gene, confers salt tolerance in transgenic tomato
SlMYB102, an R2R3-type MYB gene, confers salt tolerance in transgenic tomato
复制标题
SlMYB102，一种 R2R3 型 MYB 基因，赋予转基因番茄耐盐性
DOI：
10.1016/j.plantsci.2019.110356
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发表时间：
2020
期刊：
影响因子：
5.2
通讯作者：
Zhonghai Ren
中科院分区：
文献类型：
--
作者：
Xu Zhang;Lichen Chen;Qinghua Shi;Zhonghai Ren
Salinity threatens the productivity of tomato (Solanum lycopersicumL.). R2R3-type MYB transcription factors are important regulators in response to environmental stress. Here, we analyzed the function of the tomato R2R3-type MYB geneSlMYB102. A transcriptional activation assay showed that SlMYB102 had transactivation activity in yeast. Promoter analysis showed that multiple stress-related elements were found in the promoter ofSlMYB102. Furthermore,SlMYB102was induced by osmotic stress, particularly by salt stress. The overexpression ofSlMYB102in tomato affected multiple parameters under salinity stress. Under long-term salt stress, the degree of growth inhibition was significantly reduced in the two overexpression (OE) lines. In addition, the two OE lines maintained a better K+/Na+ratio, lower reactive oxygen species (ROS) generation (O2•−production rate and H2O2content) and lower electrolytic leakage rates than the wild type (WT). The activity of ROS scavenging enzymes including superoxide dismutase, peroxidase, catalase and ascorbate peroxidase, and the accumulation of antioxidants (ascorbic acid and glutathione) and proline was higher in the two OE lines compared with WT. The qRT-PCR analysis confirmed that the transcript abundance of many salt stress-related genes (SlSOS1,SlSOS2,SlNHX3,SlNHX4,SlHAK5,SlCPK1andSlCPK3) was upregulated in two OE lines under salt stress. Collectively, these results suggest thatSlMYB102participates in tomato tolerance through the regulation of a series of molecular and physiological processes.
