In vivo visualization using MRI T2 mapping of induced osteochondrosis and osteochondritis dissecans lesions in goats undergoing controlled exercise.
In vivo visualization using MRI T2 mapping of induced osteochondrosis and osteochondritis dissecans lesions in goats undergoing controlled exercise.
复制标题
使用 MRI T2 映射对进行受控运动的山羊进行诱导骨软骨病和骨软骨炎剥离病变进行体内可视化。
DOI：
10.1002/jor.23332
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Carlson,CathyS
中科院分区：
文献类型：
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作者：
Tóth,Ferenc;David,FrédéricH;LaFond,Elizabeth;Wang,Luning;Ellermann,JuttaM;Carlson,CathyS
亚临床骨软骨病（OC）病变的特点是骨骺生长软骨坏死，在体内可视化是必要的，以澄清这种疾病的发病机制。因此，我们的目标是证明诱导坏死的骺软骨在体内使用MRI和监测进展或解决所产生的病变。我们还通过引入控制性运动来改进山羊OC模型。在4只5日龄山羊中通过手术中断骺软骨的血管供应，以诱导股骨内侧髁的缺血性软骨坏死。术后3周开始，山羊每天进行控制性运动，直至术后6、10、11周（n= 2）处死。术后3周（n= 4）、6周（n = 4）、9周（n= 3）、11周（n= 2），用3 T磁共振扫描仪在体内获得手术组和对照组股骨的T2图。在体内MRI结果进行了验证，对MRI结果在9.4 T离体获得的三只山羊，并在所有山羊的组织学结果进行了比较。手术中断血管供应造成缺血性软骨坏死的山羊四分之三。术后3-11周，在3 T下获得的体内T2图确定了松弛时间增加的区域，与软骨坏死的离散区域一致，并在术后9（n= 1）和11（n= 2）周证实了骨化前沿的延迟进展。通过9.4 T下的离体MRI和组织学证实了体内MRI结果。使用T2图在OC的早期阶段识别临床患者的软骨坏死可能为这种情况的发病机制提供有价值的见解。© 2016骨科研究学会。出版社：Wiley Periodicals，Inc. J Orthop Res 35：868-875，2017.
In vivo visualization of subclinical osteochondrosis (OC) lesions, characterized by necrosis of epiphyseal growth cartilage, is necessary to clarify the pathogenesis of this disease. Hence, our objectives were to demonstrate induced necrosis of the epiphyseal cartilage in vivo using MRI and to monitor progression or resolution of resulting lesions. We also aimed to improve the goat model of OC by introducing controlled exercise. Vascular supply to the epiphyseal cartilage was surgically interrupted in four 5‐day‐old goats to induce ischemic cartilage necrosis in a medial femoral condyle. Starting 3 weeks postoperatively, goats underwent daily controlled exercise until euthanasia at 6, 10, 11 (n= 2) weeks postoperatively. T2maps of operated and control femora were obtained in vivo at 3 (n= 4), 6 (n= 4), 9 (n= 3), and 11 (n= 2) weeks postoperatively using a 3 T MR scanner. In vivo MRI findings were validated against MRI results obtained ex vivo at 9.4 T in three goats and compared to histological results in all goats. Surgical interruption of the vascular supply caused ischemic cartilage necrosis in three out of four goats. T2maps obtained in vivo at 3 T identified regions of increased relaxation time consistent with discrete areas of cartilage necrosis 3–11 weeks postoperatively and demonstrated delayed progression of the ossification front at 9 (n= 1) and 11 (n= 2) weeks postoperatively. In vivo MRI findings were confirmed by ex vivo MRI at 9.4 T and by histology. Identification of cartilage necrosis in clinical patients in the early stages of OC using T2maps may provide valuable insight into the pathogenesis of this condition. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:868–875, 2017.