Amphetamine and mCPP effects on dopamine and serotonin striatal in vivo microdialysates in an animal model of hyperactivity.
Amphetamine and mCPP effects on dopamine and serotonin striatal in vivo microdialysates in an animal model of hyperactivity.
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在多动动物模型中，安非他明和 mCPP 对体内微透析液中多巴胺和血清素纹状体的影响。
DOI：
10.1007/bf03033391
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发表时间：
2007
影响因子：
3.7
通讯作者：
Brus,Ryszard
中科院分区：
文献类型：
--
作者：
Nowak,Przemyslaw;Bortel,Aleksandra;Dabrowska,Joanna;Oswiecimska,Joanna;Drosik,Marzena;Kwiecinski,Adam;Opara,Józef;Kostrzewa,RichardM;Brus,Ryszard
在20世纪70年代首次描述的6-羟基多巴胺（6-OHDA）损伤大鼠中，随后发现用5，7-二羟色胺（5，7-DHT）（i. c. v.）在成年期。后一种动物模型（即，出生后3天134 μg 6-OHDA加10周75 μg 5，7-DHT;地昔帕明预处理），试图将D，L-硫酸苯丙胺（AMPH）和间氯苯基哌嗪二盐酸盐（mCPP）引起的过度运动衰减归因于神经元的特异性变化（即，在体内微透析液中）多巴胺（DA）和/或5-羟色胺（5-HT）水平。尽管纹状体组织中DA含量降低了98-99%，但与完整对照组相比，14周时DA的基线纹状体微透析液水平降低了50%或更低。当用AMPH（0.5mg/kg）激发时，DA的微透析液水平在接下来的180分钟内保持不变或略微降低（即，20 min采样），而在溶剂组和5，7-DHT（单独）损伤组中，微透析液水平分别最大升高约225%和约450%-并且持续近2 h。mCPP（1 mg/kg盐形式）急性激发对微透析液中DA、DOPAC和5-HT水平的影响很小。此外，有没有一致的变化，DA，DOPAC，和5-HT之间的微透析液水平的完整，5-HT损伤的大鼠，和DA损伤的大鼠，这可能合理地解释了衰减的hypersomotor活动。这些结果表明，有其他重要的神经化学变化所产生的AMPH和mCPP衰减hypermotorism活动，或者可能是不同的脑区域或多个脑区域的影响参与AMPH和mCPP的行为行动。
In the neonatally 6-hydroxydopamine (6-OHDA)-lesioned rat hyperlocomotor activity, first described in the 1970s, was subsequently found to be increased by an additional lesion with 5,7-dihydroxytryptamine (5,7-DHT) (i.c.v.) in adulthood. The latter animal model(i.e., 134 μg 6-OHDA at 3 d postbirth plus 75 μg 5,7-DHT at 10 weeks; desipramine pretreatments) was used in this study, in an attempt to attribute hyperlocomotor attenuation by D,L-amphet-amine sulfate (AMPH) and m-chlorophenylpi-perazine di HCl (mCPP), to specific changes in extraneuronal(i.e., in vivomicrodialysate) levels of dopamine (DA) and/or serotonin (5-HT). Despite the 98-99% reduction in striatal tissue content of DA, the baseline striatal microdialysate level of DA was reduced by 50% or less at 14 weeks, versus the intact control group. When challenged with AMPH (0.5 mg/kg), the microdialysate level of DA went either unchanged or was slightly reduced over the next 180 min(i.e., 20 min sampling), while in the vehicle group and 5,7-DHT (alone) lesioned group, the microdialysate level was maximally elevated by ~225% and ~450%, respectively - and over a span of nearly 2 h. Acute challenge with mCPP (1 mg/kg salt form) had little effect on microdialysate levels of DA, DOPAC and 5-HT. Moreover, there was no consistent change in the microdialysate levels of DA, DOPAC, and 5-HT between intact, 5-HT-lesioned rats, and DA-lesioned rats which might reasonably account for an attenuation of hyperlocomotor activity. These findings indicate that there are other important neurochemical changes produced by AMPH-and mCPP-attenuated hyperlocomotor activity, or perhaps a different brain region or multiple brain regional effects are involved in AMPH and mCPP behavioral actions.