Deep sequencing shows multiple oligouridylations are required for 3' to 5' degradation of histone mRNAs on polyribosomes.
Deep sequencing shows multiple oligouridylations are required for 3' to 5' degradation of histone mRNAs on polyribosomes.
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DOI：
10.1016/j.molcel.2014.02.027
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发表时间：
2014-03-20
期刊：
影响因子：
16
通讯作者：
Marzluff, William F.
中科院分区：
文献类型：
--
作者：
Slevin, Michael K.;Meaux, Stacie;Welch, Joshua D.;Bigler, Rebecca;de Marval, Paula L. Miliani;Su, Wei;Rhoads, Robert E.;Prins, Jan F.;Marzluff, William F.
当DNA复制在S期受到抑制时，组蛋白mRNA迅速降解，降解始于3′端茎环的寡核苷酸化。我们开发了一种定制的RNA-Seq策略来鉴定组蛋白mRNA降解中间体的3′末端。使用这种策略，我们鉴定了两种类型的寡尿苷化降解中间体：终止于茎环3 '侧不同位点的RNA，其由3' hExo初始降解产生，以及终止密码子附近和编码区内的中间体。多聚核糖体组蛋白mRNA的测序显示，降解起始于翻译mRNA的3′端至5′端，许多中间产物被加帽。外泌体相关的外切核酸酶Pml/Scl-100的敲除，而不是Dis 3L 2外切核酸酶的敲除，减缓了组蛋白mRNA的降解，这与含有PM/Scl-100的外泌体的3′至5′降解一致。敲除No-go衰变因子也减缓了组蛋白mRNA的降解，表明其在从部分降解的mRNA中去除核糖体中的作用。
Histone mRNAs are rapidly degraded when DNA replication is inhibited during S-phase with degradation initiating with oligouridylation of the stemloop at the 3′ end. We developed a customized RNA-Seq strategy to identify the 3′ termini of degradation intermediates of histone mRNAs. Using this strategy, we identified two types of oligouridylated degradation intermediates: RNAs ending at different sites of the 3′ side of the stemloop that resulted from initial degradation by 3′hExo and intermediates near the stop codon and within the coding region. Sequencing of polyribosomal histone mRNAs revealed that degradation initiates and proceeds 3′ to 5′ on translating mRNA and many intermediates are capped. Knockdown of the exosome-associated exonuclease Pml/Scl-100, but not the Dis3L2 exonuclease, slows histone mRNA degradation, consistent with 3′ to 5′ degradation by the exosome containing PM/Scl-100. Knockdown of No-go decay factors also slowed histone mRNA degradation, suggesting a role in removing ribosomes from partially degraded mRNAs.
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影响因子：
11.4
作者：
SCHARL, EC;STEITZ, JA
通讯作者：
STEITZ, JA
DOI：
10.1261/rna.042531.113
发表时间：
2014-01
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Lyons SM;Ricciardi AS;Guo AY;Kambach C;Marzluff WF
通讯作者：
Marzluff WF
影响因子：
16
作者：
Mitchell, P;Tollervey, D
通讯作者：
Tollervey, D
影响因子：
5.3
作者：
HARRIS, ME;BOHNI, R;MARZLUFF, WF
通讯作者：
MARZLUFF, WF
影响因子：
64.5
作者：
Heo, Inha;Joo, Chirlmin;Kim, V. Narry
通讯作者：
Kim, V. Narry