Retrospective study of the prevalence of high-risk human papillomaviruses among Croatian women.
Retrospective study of the prevalence of high-risk human papillomaviruses among Croatian women.
复制标题
克罗地亚妇女中高危人乳头瘤病毒流行率的回顾性研究。
作者：
Nina Milutin;I. Sabol;G. Halec;M. Matovina;M. Grce
人乳头瘤病毒(HPV)感染是宫颈癌发生的必然原因。至少有15种高危(HR)HPV类型与宫颈上皮内瘤变向宫颈癌的进展显著相关。由于以前的研究表明，不同地理区域宫颈癌中HPV的患病率不同，我们想要调查克罗地亚HPV型的患病率，特别是低丰度HPV型。采用聚合酶链式反应(PCR)方法，对2136例宫颈涂片异常的克罗地亚妇女的宫颈DNA样本进行分析，以确定是否存在HPV型特异性引物，然后用来确定低危(LR)HPV6/11型和HPV16、18、31、33、45、52和58型。在2,136例标本中，1,255例(58.8%)HPV阳性，半数以上(64.5%)阳性标本已分型，35.5%仍未分型。10.3%的病例存在多发性HPV感染。HPV16型占15.9%，其次为31、6/11、33、18、52、45和58型，分别占8.7%、7.1%、4.5%、3.8%、2.3%、1.2%和1.1%。HPV16、18、31和33型HPV16、18、31和33型从低度鳞状上皮内病变(LSIL)到高度鳞状上皮内病变(HSIL)的频率显著增加，而45、52和58型HPV45、52和58型则不明显。宫颈组织中HPV型不明率分别为19.8%和21.1%，差异无统计学意义(P>0.05)。随着年龄的增长，HPV感染率从68.5%(12~24岁)下降到38.8%(45~54岁)。但是，在55岁或55岁以上的女性中，总体患病率上升到56.6%。我们的结果表明克罗地亚人乳头瘤病毒HPV型的流行率与其他国家相似。我们建议克罗地亚的HPV阳性妇女应该通过分型密切监测HPV阳性：16、18、31、33、45、52和58。
The infection with Human papillomavirus (HPV) is the necessary cause for cervical cancer. There are at least 15 High-Risk (HR) HPV types that are significantly associated with progression of cervical intraepithelial neoplasia to cervical cancer. Since previous studies showed that the prevalence of HPV in cervical cancers varies among different geographic regions, we wanted to investigate the prevalence of HPV types in Croatia, especially low abundant HR HPV types. By means of consensus primers directed polymerase chain reaction (PCR), we analysed cervical DNA samples of 2,136 Croatian women, mostly with abnormal cervical smears, in order to detect the presence of HPV Type-specific primers were then used to determine Low-Risk (LR) HPV types 6/11 and HR HPV types 16, 18, 31, 33, 45, 52 and 58. Out of 2,136 specimens, 1,255 (58.8%) were positive for HPV More than half of positive samples were typed (64.5%) and 35.5% still remained untyped. Multiple HPV infections were found in 10.3% of the cases. The most prevalent type, including both single and multiple infections, was HPV16 with the prevalence of 15.9%, followed by HPV types 31, 6/11, 33, 18, 52, 45 and 58 with 8.7%, 7.1%, 4.5%, 3.8%, 2.3%, 1.2% and 1.1%, respectively. The significant increase of frequency from Low-grade Squamous Intraepithelial Lesions (LSIL) to High-grade Squamous Intraepithelial Lesions (HSIL) was observed for HR HPV types 16, 18, 31 and 33 but not 45, 52 and 58. The frequency of unknown HPV types was almost the same in cervical specimens of women with LSIL and those with HSIL, 19.8% and 21.1%, respectively. The prevalence of HPV infection rate decreased significantly with patient age from 68.5% (age group 12 to 24 years) to 38.8% (age group 45 to 54 years). But, in women aged 55 or older the overall prevalence increased to 56.6%. Our results indicate that prevalence of HR HPV types in Croatia is similar to other countries. We suggest that HPV positive women in Croatia should be closely monitored by typing for HR HPV types: 16, 18, 31, 33, 45, 52 and 58.